Immunolocalization of CD163+Tumor-Associated Macrophages and Symmetric Proliferation of Ki-67 as Biomarkers to Differentiate New Different Grades of Laryngeal Dysplasia
Objectives: To evaluate CD163+ tumor-associated macrophages (TAMs), Ki-67, and cyclin D1 to differentiate laryngeal dysplasia in the 2017 World Health Organization classification. Methods: Immunohistochemistry for CD163, Ki-67, and cyclin D1 was performed using paraffin-embedded specimens. CD163+ TAMs infiltrating the epithelium were estimated. Ki-67 and cyclin D1 were evaluated in four parts of the epithelium-basal, parabasal, middle third, and upper third layers. Results: In total, 133 specimens were analyzed, including low-grade dysplasia (n = 31), high-grade dysplasia (n = 49), carcinoma in situ (n = 23), and normal mucosa (n = 30). CD163+ TAMs infiltrating the epithelium were significantly higher in high-grade dysplasia than in low-grade dysplasia. In the basal layer, Ki-67+ and cyclin D1+ cells were overexpressed in high-grade dysplasia (P < .0001). The area under the curve was 0.958 for Ki-67 and 0.909 for CD163+ TAMs (P < .0001). Conclusions: CD163+ TAMs infiltrating the epithelium and Ki-67 overexpression in the basal layer may serve as biomarkers to differentiate low-grade dysplasia from high-grade dysplasia of the larynx. A symmetric proliferative pattern was observed during laryngeal carcinogenesis following Ki-67 overexpression.
基金：
Capital Health Research and Development of Special Foundation [2014-2-2053]
第一作者机构：[1]Capital Med Univ, Beijing Tongren Hosp, Beijing Key Lab Head & Neck Mol Diagnost Pathol, Dept Pathol, Dongjiaominxiang St, Beijing 100730, Peoples R China
通讯作者：
通讯机构：[1]Capital Med Univ, Beijing Tongren Hosp, Beijing Key Lab Head & Neck Mol Diagnost Pathol, Dept Pathol, Dongjiaominxiang St, Beijing 100730, Peoples R China[*1]Dept of Pathology, Beijing Key Laboratory of Head and Neck Molecular Diagnostic Pathology, Beijing Tongren Hospital, Capital Medical University, Dongjiaominxiang St, Beijing 100730, PR China
推荐引用方式(GB/T 7714)：
Su Caili,Jia Shuangshuang,Liu Honggang.Immunolocalization of CD163+Tumor-Associated Macrophages and Symmetric Proliferation of Ki-67 as Biomarkers to Differentiate New Different Grades of Laryngeal Dysplasia[J].AMERICAN JOURNAL OF CLINICAL PATHOLOGY.2018,149(1):8-16.doi:10.1093/AJCP/AQX107.
APA：
Su, Caili,Jia, Shuangshuang&Liu, Honggang.(2018).Immunolocalization of CD163+Tumor-Associated Macrophages and Symmetric Proliferation of Ki-67 as Biomarkers to Differentiate New Different Grades of Laryngeal Dysplasia.AMERICAN JOURNAL OF CLINICAL PATHOLOGY,149,(1)
MLA：
Su, Caili,et al."Immunolocalization of CD163+Tumor-Associated Macrophages and Symmetric Proliferation of Ki-67 as Biomarkers to Differentiate New Different Grades of Laryngeal Dysplasia".AMERICAN JOURNAL OF CLINICAL PATHOLOGY 149..1(2018):8-16
