Background MicroRNA (miR)-146a, as an important immune regulatory factor with an anti-inflammatory effect, plays a crucial role in regulatory T-cell (Tregs) differentiation and function in allergic rhinitis (AR). The present study aimed to investigate the regulatory mechanism employed by miR-146a to control Treg differentiation and function in AR. Methods Expression of miR-146a and STAT5b in peripheral blood mononuclear cells (PBMCs) and nasal mucosa from patients with AR was detected by qPCR and Western blotting. Tregs were quantified by flow cytometry in miR-146a knockdown or STAT5b knockdown PBMCs. FOXP3, IL-10, and TGF-beta levels were detected by Western blotting or ELISA in miR-146a knockdown or STAT5b overexpressing PBMCs, as well as in STAT5b knockdown PBMCs overexpressing miR-146a. The effect of miR-146a on STAT5b was observed by luciferase assay and knockdown experiments. Results Levels of miR146a and STAT5b in the nasal mucosa or PBMCs were significantly lower in the AR group than in the control group. There were significantly fewer Tregs in miR-146a knockdown or STAT5b knockdown PBMCs compared to control PBMCs. Expression of FOXP3, IL-10, and TGF-beta was decreased in the miR-146a knockdown group but increased in the STAT5b overexpression group. In contrast, miR-146a overexpression increased the levels of these factors, but knockdown of STAT5b significantly inhibited this effect. Luciferase assay and knockdown experiments showed that miR-146a bound directly to STAT5b. Conclusions miR-146a enhances Treg differentiation and function in AR by positively targeting STAT5b.
基金：
National Natural Science Foundation of China [82071021, 81670907]; Key R&D Project of Shandong Province [2020CXGC011302]
第一作者机构：[1]Qingdao Univ, Yantai Yuhuangding Hosp, Dept Otolaryngol Head & Neck Surg, Yantai 264000, Peoples R China
共同第一作者：
通讯作者：
通讯机构：[1]Qingdao Univ, Yantai Yuhuangding Hosp, Dept Otolaryngol Head & Neck Surg, Yantai 264000, Peoples R China[2]Capital Med Univ, Beijing Tongren Hosp, Dept Otolaryngol Head & Neck Surg, 17 Hou Gou Hu Tong, Beijing 100005, Peoples R China[3]Beijing Inst Otolaryngol, Beijing Key Lab Nasal Dis, Beijing, Peoples R China[*1]Department of Otolaryngology, Head and Neck Surgery, Yantai Yuhuangding Hospital, Qingdao University, Yantai 264000, China.[*2]Department of Otolaryngology, Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, No. 17, Hou Gou Hu Tong, Dong Cheng District, 100005 Beijing, China.
推荐引用方式(GB/T 7714)：
Zhang Yu,Yang Yujuan,Guo Jing,et al.miR-146a enhances regulatory T-cell differentiation and function in allergic rhinitis by targeting STAT5b[J].ALLERGY.2022,77(2):550-558.doi:10.1111/all.15163.
APA：
Zhang, Yu,Yang, Yujuan,Guo, Jing,Cui, Limei,Yang, Liping...&Song, Xicheng.(2022).miR-146a enhances regulatory T-cell differentiation and function in allergic rhinitis by targeting STAT5b.ALLERGY,77,(2)
MLA：
Zhang, Yu,et al."miR-146a enhances regulatory T-cell differentiation and function in allergic rhinitis by targeting STAT5b".ALLERGY 77..2(2022):550-558
医学