机构：[1]Department of Orthopedics, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, 600 Yishan Road, Shanghai 200233, China[2]Joint Department of Biomedical Engineering, The University of North Carolina at Chapel Hill and North Carolina State University, Chapel Hill, NC, USA[3]Department of Orthopedics, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, 1111 XianXia Road, Shanghai 200336, China
Background: Osteoporosis is a chronic condition affecting patients' morbidity and mortality and represents a big socioeconomic burden. Because stem cells can proliferate and differentiate into bone-forming cells, stem cell therapy for osteoporosis has been widely studied. However, cells as a live drug face multiple challenges because of their instability during preservation and transportation. In addition, cell therapy has potential adverse effects such as embolism, tumorigenicity, and immunogenicity. Results: Herein, we sought to use cell-mimicking and targeted therapeutic nanoparticles to replace stem cells. We fabricated nanoparticles (NPs) using polylactic-co-glycolic acid (PLGA) loaded with the secretome (Sec) from mesenchymal stem cells (MSCs) to form MSC-Sec NPs. Furthermore, we cloaked the nanoparticles with the membranes from C-X-C chemokine receptor type 4 (CXCR4)-expressing human microvascular endothelial cells (HMECs) to generate MSC-Sec/CXCR4 NP. CXCR4 can target the nanoparticles to the bone microenvironment under osteoporosis based on the CXCR4/SDF-1 axis. Conclusions: In a rat model of osteoporosis, MSC-Sec/CXCR4 NP were found to accumulate in bone, and such treatment inhibited osteoclast differentiation while promoting osteogenic proliferation. In addition, our results showed that MSC-Sec/CXCR4 NPs reduce OVX-induced bone mass attenuation in OVX rats.
基金：
This work was supported by NC State University College
of Veterinary Medicine. Project is also sponsored by National Natural Science
Foundation of China (No. 81772426 to B. Zhong, 81974330 to C. Zhang,
81501897 to W. Xu) and Shanghai Committee of Science and Technology (No.
20ZR1451800 to W. Xu).
第一作者机构：[1]Department of Orthopedics, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, 600 Yishan Road, Shanghai 200233, China[2]Joint Department of Biomedical Engineering, The University of North Carolina at Chapel Hill and North Carolina State University, Chapel Hill, NC, USA
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Zhang Chi,Zhang Wei,Zhu Dashuai,et al.Nanoparticles functionalized with stem cell secretome and CXCR4-overexpressing endothelial membrane for targeted osteoporosis therapy[J].JOURNAL OF NANOBIOTECHNOLOGY.2022,20(1):doi:10.1186/s12951-021-01231-6.
APA：
Zhang, Chi,Zhang, Wei,Zhu, Dashuai,Li, Zhenhua,Wang, Zhenzhen...&Zhong, Biao.(2022).Nanoparticles functionalized with stem cell secretome and CXCR4-overexpressing endothelial membrane for targeted osteoporosis therapy.JOURNAL OF NANOBIOTECHNOLOGY,20,(1)
MLA：
Zhang, Chi,et al."Nanoparticles functionalized with stem cell secretome and CXCR4-overexpressing endothelial membrane for targeted osteoporosis therapy".JOURNAL OF NANOBIOTECHNOLOGY 20..1(2022)
生物学