Cell division control protein 42 homolog (Cdc42), one of the most characteristic members of the Rho protein family, is required for multiple aspects of dendritic morphogenesis. However, the proteins mediating the regulatory effects of Cdc42 activity on neuronal morphology are largely unknown. Cdc42 effector protein-4 (CEP4) was identified to be a binding partner of Rho GTPase 4 and is ubiquitously expressed in all adult tissues. However, the physiological function of CEP4 in neurons is unknown. In the present study, immunofluorescence and western blot analysis were conducted, revealing that CEP4 is highly expressed in the brain, and that the expression of CEP4 is gradually increased during neurodevelopment. Knockdown of CEP4 with short hairpin RNA suppressed dendrite growth, whereas overexpression of wild-type CEP4 promoted dendrite growth in primary isolated mouse hippocampal neurons. Collectively, these results indicated an important role for CEP4 in dendrite growth in hippocampal neurons.
基金：
National Natural Science Foundation of China for Young ScholarsNational Natural Science Foundation of China (NSFC) [82102307]; Biomedical and Engineering Cross Youth Fund of Shanghai Jiao Tong University [YG2021QN43]
第一作者机构：[1]Soochow Univ, Affiliated Hosp 1, Dept Neurosurg, 188 Shizi St, Suzhou 215006, Jiangsu, Peoples R China[2]Shanghai Univ Med & Hlth Sci, Jiading Dist Cent Hosp, Dept Neurosurg, Shanghai 201899, Peoples R China
共同第一作者：
通讯作者：
通讯机构：[1]Soochow Univ, Affiliated Hosp 1, Dept Neurosurg, 188 Shizi St, Suzhou 215006, Jiangsu, Peoples R China[6]Shanghai Jiao Tong Univ, Sch Med, Xinhua Hosp, Dept Emergency, 1665 Kongjiang Rd, Shanghai 200092, Peoples R China[*1]Department of Emergency, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, 1665 Kongjiang Road, Yangpu, Shanghai 200092, P.R. China[*2]Department of Neurosurgery, The First Affiliated Hospital of Soochow University, 188 Shizi Street, Gusu, Suzhou, Jiangsu 215006, P.R. China
推荐引用方式(GB/T 7714)：
Hou Lei,Wang Lufeng,Zhao Zhijie,et al.Regulation of dendrite growth by Cdc42 effector protein-4 in hippocampal neurons in vitro[J].MOLECULAR MEDICINE REPORTS.2022,25(4):doi:10.3892/mmr.2022.12644.
APA：
Hou, Lei,Wang, Lufeng,Zhao, Zhijie,Xu, Wei,Wang, Yang&Cui, Gang.(2022).Regulation of dendrite growth by Cdc42 effector protein-4 in hippocampal neurons in vitro.MOLECULAR MEDICINE REPORTS,25,(4)
MLA：
Hou, Lei,et al."Regulation of dendrite growth by Cdc42 effector protein-4 in hippocampal neurons in vitro".MOLECULAR MEDICINE REPORTS 25..4(2022)
