Quercetin Protects H9c2 Cardiomyocytes against Oxygen-Glucose Deprivation/Reoxygenation-Induced Oxidative Stress and Mitochondrial Apoptosis by Regulating the ERK1/2/DRP1 Signaling Pathway
机构：[1]Department of Neurology, Wuhan THird Hospital & Tongren Hospital of Wuhan University, Wuhan 430074, China[2]Department of Cardiology, Wuhan THird Hospital & Tongren Hospital of Wuhan University, Wuhan 430074, China[3]Department of Forensic Science, Changsha 410013, China[4]Department of Clinical Medicine, Jianghan University, Wuhan 430056, China
Reperfusion of blood flow during ischemic myocardium resuscitation induces ischemia/reperfusion (I/R) injury. Oxidative stress has been identified as a major cause in this process. Quercetin (QCT) is a member of the flavonoid family that exerts antioxidant effects. The aim of this study was to investigate the preventive effects of QCT on I/R injury and its underlying mechanism. To this end, H9c2 cardiomyocytes were treated with different concentrations of QCT (10, 20, and 40 mu M) and subsequently subjected to oxygen-glucose deprivation/reperfusion (OGD/R) administration. The results indicated that OGD/R-induced oxidative stress, apoptosis, and mitochondrial dysfunction in H9c2 cardiomyocytes were aggravated following 40 mu M QCT treatment and alleviated following the administration of 10 and 20 mu M QCT prior to OGD/R treatment. In addition, OGD/R treatment inactivated ERK1/2 signaling activation. The effect was mitigated using 10 and 20 mu M QCT prior to OGD/R treatment. In conclusion, these results suggested that low concentrations of QCT might alleviate I/R injury by suppressing oxidative stress and improving mitochondrial function through the regulation of ERK1/2-DRP1 signaling, providing a potential candidate for I/R injury prevention.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81871088]; Hubei Province Natural Science Fund [2020CFB660]; Applied Basic Research Program of Wuhan Municipal Bureau of Science and Technology [2019020701011473, 2020020601012305]; Wuhan Municipal Population and Family Planning Commission Foundation [WX16C03, WX20Q34]; Hubei Province Health and Family Planning Scientific Research Project [WJ2019M006]
第一作者机构：[1]Department of Neurology, Wuhan THird Hospital & Tongren Hospital of Wuhan University, Wuhan 430074, China
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推荐引用方式(GB/T 7714)：
Li Fen,Li Dongsheng,Tang Shifan,et al.Quercetin Protects H9c2 Cardiomyocytes against Oxygen-Glucose Deprivation/Reoxygenation-Induced Oxidative Stress and Mitochondrial Apoptosis by Regulating the ERK1/2/DRP1 Signaling Pathway[J].EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE.2021,2021:doi:10.1155/2021/7522175.
APA：
Li, Fen,Li, Dongsheng,Tang, Shifan,Liu, Jianguang,Yan, Jie...&Yan, Xisheng.(2021).Quercetin Protects H9c2 Cardiomyocytes against Oxygen-Glucose Deprivation/Reoxygenation-Induced Oxidative Stress and Mitochondrial Apoptosis by Regulating the ERK1/2/DRP1 Signaling Pathway.EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE,2021,
MLA：
Li, Fen,et al."Quercetin Protects H9c2 Cardiomyocytes against Oxygen-Glucose Deprivation/Reoxygenation-Induced Oxidative Stress and Mitochondrial Apoptosis by Regulating the ERK1/2/DRP1 Signaling Pathway".EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE 2021.(2021)
