机构：[1]School of Biomedical Engineering, Capital Medical University, Beijing, 100069, China[2]Beijing Key Laboratory of Fundamental Research on Biomechanics in Clinical Application, Capital Medical University, Beijing, 100069, China[3]CAS Key Laboratory of Molecular Imaging, The State Key Laboratory of Management and Control for Complex Systems, Institute of Automation, Chinese Academy of Sciences, Beijing, 100190, China[4]School of Electronics and Information Engineering and the State Key Laboratory of Radiation Medicine and Protection, Soochow University, Jiangsu, 215006, China[5]Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing, 100730, China首都医科大学附属北京同仁医院临床科室眼科眼底科[6]NIHR Moorfields Biomedical Research Centre, Moorfields Eye Hospital, London, EC1 V 2PD, UK
Anti-vascular endothelial growth factor (anti-VEGF) therapy is effective for reducing the severity level of diabetic retinopathy (DR). However, it is difficult to determine the in vivo spatial and temporal expression of VEGF in the DR retina at an early stage. Here, we report a quantitatively fluorescence molecular imaging and image analysis method by creating a VEGF targeted fluorescence imaging probe, which can potentially detect and predict anti-VEGF treatment response. Moreover, the ex vivo multiscale fluorescence imaging demonstrated the spatial correlation between VEGF relative expression and vascular abnormalities in two and three dimensions. It revealed that VEGF was mainly abnormally expressed at the bifurcation of the microvessels, which advances the knowledge of the DR progression by molecular fluorescence imaging. Our study has the potential to achieve early detection of DR disease, provide more insight into understanding anti-VEGF treatment, and may help stratify patients based on the molecular imaging of retinal VEGF.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [61771326, 61827809]; Science and Technology Project of Beijing Municipal Commission of Education [KM202010025026]
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2020]版：
大类|2 区医学
小类|2 区生化研究方法2 区光学2 区核医学
最新[2025]版：
大类|3 区医学
小类|2 区生化研究方法3 区光学3 区核医学
JCR分区：
出版当年[2019]版：
Q1BIOCHEMICAL RESEARCH METHODSQ1OPTICSQ1RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING
最新[2023]版：
Q2BIOCHEMICAL RESEARCH METHODSQ2OPTICSQ2RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING
第一作者机构：[1]School of Biomedical Engineering, Capital Medical University, Beijing, 100069, China[2]Beijing Key Laboratory of Fundamental Research on Biomechanics in Clinical Application, Capital Medical University, Beijing, 100069, China[3]CAS Key Laboratory of Molecular Imaging, The State Key Laboratory of Management and Control for Complex Systems, Institute of Automation, Chinese Academy of Sciences, Beijing, 100190, China
共同第一作者：
通讯作者：
通讯机构：[1]School of Biomedical Engineering, Capital Medical University, Beijing, 100069, China[2]Beijing Key Laboratory of Fundamental Research on Biomechanics in Clinical Application, Capital Medical University, Beijing, 100069, China
推荐引用方式(GB/T 7714)：
Zhang Lu,Ding Yunhe,Chen Xinjian,et al.In vivo fluorescence molecular imaging of the vascular endothelial growth factor in rats with early diabetic retinopathy[J].BIOMEDICAL OPTICS EXPRESS.2021,12(11):7185-7198.doi:10.1364/BOE.439446.
APA：
Zhang, Lu,Ding, Yunhe,Chen, Xinjian,Xiang, Dehui,Shi, Fei...&Tian, Bei.(2021).In vivo fluorescence molecular imaging of the vascular endothelial growth factor in rats with early diabetic retinopathy.BIOMEDICAL OPTICS EXPRESS,12,(11)
MLA：
Zhang, Lu,et al."In vivo fluorescence molecular imaging of the vascular endothelial growth factor in rats with early diabetic retinopathy".BIOMEDICAL OPTICS EXPRESS 12..11(2021):7185-7198
