Regeneration of Subcutaneous Cartilage in a Swine Model Using Autologous Auricular Chondrocytes and Electrospun Nanofiber Membranes Under Conditions of Varying Gelatin/PCL Ratios
机构：[1]Department of Plastic and Reconstructive Surgery, Shanghai 9th People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering, Shanghai Stem Cell Institute, Shanghai, China,[2]Department of Dermatology, Shanghai 9th People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China,[3]Department of Cosmetic Surgery, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China,[4]Department of Orthopaedics, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, China,[5]National Tissue Engineering Center of China, Shanghai, China[6]Research Institute of Plastic Surgery, Weifang Medical College, Weifang, China
The scarcity of ideal biocompatible scaffolds makes the regeneration of cartilage in the subcutaneous environment of large animals difficult. We have previously reported the successful regeneration of good-quality cartilage in a nude mouse model using the electrospun gelatin/polycaprolactone (GT/PCL) nanofiber membranes. The GT/PCL ratios were varied to generate different sets of membranes to conduct the experiments. However, it is unknown whether these GT/PCL membranes can support the process of cartilage regeneration in an immunocompetent large animal model. We seeded swine auricular chondrocytes onto different GT/PCL nanofiber membranes (GT:PCL = 30:70, 50:50, and 70:30) under the sandwich cell-seeding mode. Prior to subcutaneously implanting the samples into an autologous host, they were cultured in vitro over a period of 2 weeks. The results revealed that the nanofiber membranes with different GT/PCL ratios could support the process of subcutaneous cartilage regeneration in an autologous swine model. The maximum extent of homogeneity in the cartilage tissues was achieved when the G5P5 (GT: PC = 50: 50) group was used for the regeneration of cartilage. The formed homogeneous cartilage tissues were characterized by the maximum cartilage formation ratio. The extents of the ingrowth of the fibrous tissues realized and the extents of infiltration of inflammatory cells achieved were found to be the minimum in this case. Quantitative analyses were conducted to determine the wet weight, cartilage-specific extracellular matrix content, and Young's modulus. The results indicated that the optimal extent of cartilage formation was observed in the G5P5 group. These results indicated that the GT/PCL nanofiber membranes could serve as a potential scaffold for supporting subcutaneous cartilage regeneration under clinical settings. An optimum GT/PCL ratio can promote cartilage formation.
基金：
National Key Research and Development Program of China [2017YFC1103900]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81871502, 81974291, 81701843, 31700837, 81671837]; Shanghai Collaborative Innovation Program on Regenerative Medicine and Stem Cell Research [2019CXJQ01]; Program of Shanghai Academic/Technology Research Leader [19XD1431100]; Clinical Research Plan of SHDC [SHDC2020CR 2045B]; Cross-disciplinary Research Fund of Shanghai Ninth People's Hospital, Shanghai JiaoTong University School of Medicine [JYJC201901, JYJC202105]
第一作者机构：[1]Department of Plastic and Reconstructive Surgery, Shanghai 9th People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering, Shanghai Stem Cell Institute, Shanghai, China,[2]Department of Dermatology, Shanghai 9th People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China,
共同第一作者：
通讯作者：
通讯机构：[1]Department of Plastic and Reconstructive Surgery, Shanghai 9th People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering, Shanghai Stem Cell Institute, Shanghai, China,[5]National Tissue Engineering Center of China, Shanghai, China[6]Research Institute of Plastic Surgery, Weifang Medical College, Weifang, China
推荐引用方式(GB/T 7714)：
Zheng Rui,Wang Xiaoyun,Xue Jixin,et al.Regeneration of Subcutaneous Cartilage in a Swine Model Using Autologous Auricular Chondrocytes and Electrospun Nanofiber Membranes Under Conditions of Varying Gelatin/PCL Ratios[J].FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY.2021,9:doi:10.3389/fbioe.2021.752677.
APA：
Zheng, Rui,Wang, Xiaoyun,Xue, Jixin,Yao, Lin,Wu, Gaoyang...&Zhou, Guangdong.(2021).Regeneration of Subcutaneous Cartilage in a Swine Model Using Autologous Auricular Chondrocytes and Electrospun Nanofiber Membranes Under Conditions of Varying Gelatin/PCL Ratios.FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY,9,
MLA：
Zheng, Rui,et al."Regeneration of Subcutaneous Cartilage in a Swine Model Using Autologous Auricular Chondrocytes and Electrospun Nanofiber Membranes Under Conditions of Varying Gelatin/PCL Ratios".FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY 9.(2021)
