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Cytokeratin 13 Is a New Biomarker for the Diagnosis of Limbal Stem Cell Deficiency.

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机构： [1]Stein Eye Institute, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA [2]Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Key Laboratory of Ophthalmology and Visual Sciences, Beijing, China [3]Department of Ophthalmology, Eye, Ear, Nose & Throat Hospital of Fudan University, Shanghai, China [4]Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA [5]Department of Medicine, Statistic Core-General Internal Medicine and Health Service Research, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA [6]Molecular Biology Institute, University of California, Los Angeles, Los Angeles, CA.

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关键词： limbal stem cell deficiency limbal stem cell impression cytology cytokeratin 13 diagnosis

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The purpose of this study was to evaluate the expression of cytokeratin (K) 13 on the corneal surface and to validate its application in the diagnosis of limbal stem cell deficiency (LSCD).This prospective comparative study included 26 corneal impression cytology (IC) specimens from patients diagnosed with LSCD. Twenty-three IC specimens from normal donors served as controls. K12 and K13 expression were detected on the IC specimens by immunohistochemistry study. The number of K12+ or K13+ cells in all areas of the IC was quantified using ImageJ software.The epithelial cells harvested from IC specimens from control corneas were all K12+. In eyes with LSCD, K13+ and K12+/K13+ cells accounted for 93.8% and 2.6%, respectively, in the cornea. In eyes with sectoral LSCD, the median number of K13+ cells in the clinically affected area was higher than that in the unaffected area (810.0 vs. 115.0 cells/mm2; P < 0.001). No significant correlation was found between the LSCD severity and the number of K12+ cells (r = -0.284, P = 0.16) or K13+ cells (r = -0.011, P = 0.95). The presence of at least 16 K13+ cells/mm2 was suggestive of LSCD.Identification of K13+ cells on IC specimens provides a simple and reliable method to detect conjunctival epithelial cells on the cornea. K13 is a marker for diagnosing LSCD and localizing the involved area in sectoral LSCD.Copyright © 2021 Wolters Kluwer Health, Inc. All rights reserved.

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出版当年[2021]版：

大类 | 3 区医学

小类 | 3 区眼科学

最新[2025]版：

大类 | 4 区医学

小类 | 4 区眼科学

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出版当年[2020]版：

Q3 OPHTHALMOLOGY

最新[2023]版：

Q2 OPHTHALMOLOGY

影响因子： 1.9 最新[2023版] 2.3 最新五年平均 2.651 出版当年[2020版] 2.774 出版当年五年平均 2.215 出版前一年[2019版] 3.152 出版后一年[2021版]

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第一作者机构： [1]Stein Eye Institute, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA [2]Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Key Laboratory of Ophthalmology and Visual Sciences, Beijing, China

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通讯作者：

通讯机构： [1]Stein Eye Institute, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA [6]Molecular Biology Institute, University of California, Los Angeles, Los Angeles, CA. [*1]Stein Eye Institute, David Geffen School of Medicine at UCLA, 100 Stein Plaza, Los Angeles, CA 90095

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