机构：[1]Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering, Shanghai, PR China.[2]National Tissue Engineering Center of China, Shanghai, PR China.[3]Research Institute of Plastic Surgery, Weifang Medical University, Weifang, Shandong, PR China.[4]Department of Plastic Surgery, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering, Shanghai, PR China.[5]Department of Hand Surgery, Ningbo Sixth Hospital, Ningbo, Zhejiang, PR China.[6]Institute of Regenerative Medicine and Orthopedics, Institutes of Health Central Plain, Xinxiang Medical University, Xinxiang, Henan, PR China.
Tissue engineering is a promising strategy for cartilage defect repair. However, autologous cartilage regeneration is limited by additional trauma to the donor site and a long in vitro culture period. Alternatively, allogenic cartilage regeneration has attracted attention because of the unique advantages of an abundant donor source and immediate supply, but it will cause immune rejection responses (IRRs), especially in immunocompetent large animals. Therefore, a universal technique needs to be established to overcome IRRs for allogenic cartilage regeneration in large animals. In the current study, we developed a hybrid synthetic-natural electrospun thermoplastic polyurethane/gelatin (TPU/GT) semipermeable membrane to explore the feasibility of stable allogenic cartilage regeneration by an immunoisolation strategy. In vitro results demonstrated that the rationally designed electrospun TPU/GT membranes had ideal biocompatibility, semipermeability, and an immunoisolation function. In vivo results further showed that the semipermeable membrane (SPM) efficiently blocked immune cell attack, decreased immune factor production and cell apoptosis of the regenerated allogenic cartilage. Importantly, TPU/GT-encapsulated cartilage-sheet constructs achieved stable allogenic cartilage regeneration in a goat model. The current study provides a novel strategy for allogenic cartilage regeneration and supplies a new cartilage donor source to repair various cartilage defects. This article is protected by copyright. All rights reserved.This article is protected by copyright. All rights reserved.
基金：
National Key Research and Development Program
of China (2017YFC1103900 and 2018YFC1105800), the National Natural
Science Foundation of China (81871502 and 81701843), the Shanghai
Collaborative Innovation Program on Regenerative Medicine and Stem
Cell Research (2019CXJQ01), the Key Research and Development Program
of Henan province (221111310100), Major Science and Technology
Projects of Xinxiang City (21ZD006), the Clinical Research Plan of
SHDC (SHDC2020CR2045B), Shanghai Municipal Key Clinical Specialty
(shslczdzk06601), and Zhejiang Medical and Health Science and Technology
Project (2019KY624).
第一作者机构：[1]Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering, Shanghai, PR China.[2]National Tissue Engineering Center of China, Shanghai, PR China.
共同第一作者：
通讯作者：
通讯机构：[1]Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering, Shanghai, PR China.[2]National Tissue Engineering Center of China, Shanghai, PR China.[3]Research Institute of Plastic Surgery, Weifang Medical University, Weifang, Shandong, PR China.[4]Department of Plastic Surgery, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering, Shanghai, PR China.[5]Department of Hand Surgery, Ningbo Sixth Hospital, Ningbo, Zhejiang, PR China.[6]Institute of Regenerative Medicine and Orthopedics, Institutes of Health Central Plain, Xinxiang Medical University, Xinxiang, Henan, PR China.
推荐引用方式(GB/T 7714)：
Huo Yingying,Bai Baoshuai,Zheng Rui,et al.In Vivo Stable Allogenic Cartilage Regeneration in A Goat Model Based on Immunoisolation Strategy Using Electrospun Semipermeable Membranes[J].ADVANCED HEALTHCARE MATERIALS.2023,12(13):doi:10.1002/adhm.202203084.
APA：
Huo Yingying,Bai Baoshuai,Zheng Rui,Sun Yuyan,Yu Yao...&Wang Xiaoyun.(2023).In Vivo Stable Allogenic Cartilage Regeneration in A Goat Model Based on Immunoisolation Strategy Using Electrospun Semipermeable Membranes.ADVANCED HEALTHCARE MATERIALS,12,(13)
MLA：
Huo Yingying,et al."In Vivo Stable Allogenic Cartilage Regeneration in A Goat Model Based on Immunoisolation Strategy Using Electrospun Semipermeable Membranes".ADVANCED HEALTHCARE MATERIALS 12..13(2023)
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