The method of establishing fingerprint profiles of chemical characteristics of herbal medicines is currently one of the most effective methods recognized internationally for controlling the quality of herbal or natural medicines. This work systematically examines geographical differences using the bark and leaves of Eucommia ulmoide as the object of study. Electrochemical fingerprinting and Fourier transform infrared (FTIR) fingerprinting were used to obtain information on different samples. We established a common pattern for the profiles. The results showed that the differences in response positions and intensities of the aspiration fingerprint profiles of the samples from various origins were more obvious and could effectively identify the origins. We combined this with principal component analysis, and the results showed that the differences in chemical composition and content of the samples were correlated with the geographical locations in which they were located. The com-parative analysis of the second-order derivative spectra further illustrated the differences in chemical compo-sition and content between samples of different origins.