机构：[1]Department of Neurology, First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, 215006, China.[2]Department of Neurology, Tongren Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200336, China.[3]Hongqiao International Institute of Medicine, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200336, China.[4]Department of Anesthesiology, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200336, China.[5]Institute of Clinical Immunology, Jiangsu Key Laboratory of Clinical Immunology, First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, 215006, China.
Parkinson's disease (PD) is a serious neurodegenerative disorder wherein changes in metabolites related to lipids, glutathione, and energy metabolism occur. Currently, metabolite changes in PD have been reported, yet their role in the prognosis of disease remains poorly understood. Functional metabolites can be used to diagnose diseases, especially PD, and can exert neuroprotective effects. This study used a PD animal model and a lipopolysaccharide (LPS)-mediated inflammatory response model (using the BV-2 mouse microglial cell line) to identify functional metabolites that can identify important metabolic disorders during PD, and comprehensively evaluated their profiles using a metabolomics-based approach. Our results showed that co-treatment with myristic acid and heptadecanoic acid downregulated the expression of interleukin (IL)-1β, IL-6, and tumor necrosis factor-α in BV-2 cells. Additionally, myristic acid and 10 μM heptadecanoic acid significantly inhibited the LPS-induced inflammatory response through the nuclear factor-κB pathway in BV-2 microglial cells, which provides a potential approach for PD treatment. Myristic acid and heptadecanoic acid were the active metabolites found by active metabolomics technology, but at present, there is no research report about their function for PD treatment, and our findings offer a novel research strategy for PD diagnosis and treatment.
基金：
The grant from the funds of Shanghai Municipal Health
Commission (Project No. 20214Y0432), the natural science
foundation of Jiangsu Province (BK20211075), and Shanghai
Changning District Municipal Health Commission (Project No.
20214Y002) supported this research.
第一作者机构：[1]Department of Neurology, First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, 215006, China.[2]Department of Neurology, Tongren Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200336, China.
通讯作者：
通讯机构：[1]Department of Neurology, First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, 215006, China.[3]Hongqiao International Institute of Medicine, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200336, China.[4]Department of Anesthesiology, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200336, China.[5]Institute of Clinical Immunology, Jiangsu Key Laboratory of Clinical Immunology, First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, 215006, China.
推荐引用方式(GB/T 7714)：
Huang Qiong,Chen Chunyan,Zhang Zhongxiao,et al.Anti-inflammatory effects of myristic acid mediated by the NF-κB pathway in lipopolysaccharide-induced BV-2 microglial cells[J].MOLECULAR OMICS.2023,19(9):726-734.doi:10.1039/d3mo00063j.
APA：
Huang Qiong,Chen Chunyan,Zhang Zhongxiao&Xue Qun.(2023).Anti-inflammatory effects of myristic acid mediated by the NF-κB pathway in lipopolysaccharide-induced BV-2 microglial cells.MOLECULAR OMICS,19,(9)
MLA：
Huang Qiong,et al."Anti-inflammatory effects of myristic acid mediated by the NF-κB pathway in lipopolysaccharide-induced BV-2 microglial cells".MOLECULAR OMICS 19..9(2023):726-734
