Understanding the cellular composition and trajectory of human tooth development is valuable for dentistry and stem cell engineering research. Previous single-cell studies have focused on mature human teeth and developing mouse teeth, but the cell landscape of human embryonic dental development is still unknown. In this study, tooth germ tissues were collected from aborted foetus (17-24 weeks) for single-cell RNA sequence and spatial transcriptome analysis. The cells were classified into seven subclusters of epithelium, and seven clusters of mesenchyme, as well as other cell types such as Schwann cell precursor and pericyte. For epithelium, the stratum intermedium branch and the ameloblast branch diverged from the same set of outer enamel-inner enamel-ALCAM+ epithelial cell lineage, but their spatial distribution of two branches was not clearly distinct. This trajectory received spatially adjacent regulation signals from mesenchyme and pericyte, including JAG1 and APP. The differentiation of pulp cell and pre-odontoblast showed four waves of temporally distinct gene expression, which involved regulation networks of LHX9, DLX5 and SP7, and these genes were regulated by upstream ligands such as the BMP family. This provides a reference landscape for the research on early human tooth development, covering different spatial structures and developmental periods. This study integrated single-cell transcriptome and spatial transcriptome to depict the developmental trajectory of epithelial and mesenchymal cells in human foetal tooth. image
基金:
Research Fund of Shanghai Tongren Hospital,
Shanghai Jiaotong University School of
Medicine, Grant/Award Numbers:
TRYJ2021JC14, TRKYRC-xx202207
第一作者机构:[1]Shanghai Jiao Tong Univ, Sch Med, Tongren Hosp, Dept Stomatol, 1111 XianXia Rd, Shanghai 200336, Peoples R China
通讯作者:
推荐引用方式(GB/T 7714):
Shi Yueqi,Yu Yejia,Li Jutang,et al.Spatiotemporal cell landscape of human embryonic tooth development[J].CELL PROLIFERATION.2024,57(9):doi:10.1111/cpr.13653.
APA:
Shi, Yueqi,Yu, Yejia,Li, Jutang,Sun, Shoufu,Han, Li...&Wei, Wenjia.(2024).Spatiotemporal cell landscape of human embryonic tooth development.CELL PROLIFERATION,57,(9)
MLA:
Shi, Yueqi,et al."Spatiotemporal cell landscape of human embryonic tooth development".CELL PROLIFERATION 57..9(2024)