个人科研网厅 | | 帮助
高级检索
当前位置： 首页 > 详情页

Caffeic Acid Reduces Ferroptosis to Dampen Inflammation of Keratinocytes in Psoriasis by Inhibiting EGR1-induced Transcription Activation of CHAC1

| 认领 | 导出 | |

文献详情

资源类型：
WOS体系：
Pubmed体系：

收录情况： ◇ SCIE

作者：
Li Yadi[1] * ; Zhou Xiaoqian[1] ; Yang Zhusheng[1] ; Zhang Lichao[1] ; Yang Xiumin[1] ; Wei Aihua[1,*1] ;
机构： [1]Department of Dermatology, Beijing Tongren Hospital, Capital Medical University, No.1 Dongjiaomin Lane, Dongcheng District, Beijing, 100730, China.
出处：
DOI： 10.2174/0115665240296144240419063309
ISSN：

关键词： Caffeic acid psoriasis keratinocytes EGR1 CHAC1

摘要：
Ferroptosis of keratinocytes is closely associated with amplification of skin inflammation in psoriasis. This study focuses on unlocking the role of caffeic acid (CA), a polyphenol compound, in keratinocyte ferroptosis and understanding the underlying mechanistic basis.The interaction between early growth response protein 1 (EGR1) and chac glutathione specific γ‑glutamylcyclotransferase 1 (CHAC1) was predicted by bioinformatics and validated via chromatin immunoprecipitation and dual-luciferase reported assays. Their expressions in primary human epidermal keratinocytes were altered by transfection of EGR1/CHAC1 overexpression or knockdown plasmids, and then keratinocytes were followed by CA treatment and Erastin (ferroptosis inducer). Keratinocyte viability was determined by CCK-8 assay, and the ferroptotic effect was evaluated using colorimetric assay and flow cytometry. Proinflammatory cytokine secretion by keratinocytes was detected via ELISA. Expressions of EGR1 and CHAC1 in keratinocytes were analyzed by qRT-PCR or Western blot.Increased expressions of EGR1 and CHAC1 were detected in keratinocytes with Erastin treatment. CA (100 μM) antagonized Erastin (10 μM)-induced decrease in viability, increases in EGR1 and CHAC1 expressions, upregulation of MDA, ROS, and Fe2+, downregulation of GSH and SOD, and secretion of proinflammatory cytokines from keratinocytes. EGR1 overexpression potentiated Erastin-induced effects. Moreover, EGR1 overexpression and CA mutually counteracted their effects on Erastin-induced keratinocytes. EGR1 transcriptionally activated and positively regulated CHAC1. The above Erastin-induced effects were neutralized by EGR1 knockdown but potentiated by CHAC1 overexpression. Moreover, EGR1 knockdown and CHAC1 overexpression reversed each other's effects.CA reduces ferroptosis by inhibiting EGR1-induced activation of CHAC1 to dampen inflammation of keratinocytes in psoriasis. This study providing new compounds and candidate targets for the clinical treatment of psoriasis.Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.

语种：
外文
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2023]版：
大类 | 4 区 医学
小类 | 4 区 医学：研究与实验
最新[2023]版：
大类 | 4 区 医学
小类 | 4 区 医学：研究与实验
JCR分区：
出版当年[2022]版：
Q3 MEDICINE, RESEARCH & EXPERIMENTAL
最新[2023]版：
Q3 MEDICINE, RESEARCH & EXPERIMENTAL

影响因子： 最新[2023版] 最新五年平均 出版当年[2022版] 出版当年五年平均 出版前一年[2021版] 出版后一年[2023版]

第一作者：
第一作者机构： [1]Department of Dermatology, Beijing Tongren Hospital, Capital Medical University, No.1 Dongjiaomin Lane, Dongcheng District, Beijing, 100730, China.
通讯作者：
通讯机构： [1]Department of Dermatology, Beijing Tongren Hospital, Capital Medical University, No.1 Dongjiaomin Lane, Dongcheng District, Beijing, 100730, China. [*1]Department of Dermatology, Beijing Tongren Hospital, Capital Medical University,No.1 Dongjiaomin Lane, Dongcheng District, Beijing, 100730, China
推荐引用方式(GB/T 7714)：
Li Yadi,Zhou Xiaoqian,Yang Zhusheng,et al.Caffeic Acid Reduces Ferroptosis to Dampen Inflammation of Keratinocytes in Psoriasis by Inhibiting EGR1-induced Transcription Activation of CHAC1[J].Current Molecular Medicine.2024,doi:10.2174/0115665240296144240419063309.
APA：
Li Yadi,Zhou Xiaoqian,Yang Zhusheng,Zhang Lichao,Yang Xiumin&Wei Aihua.(2024).Caffeic Acid Reduces Ferroptosis to Dampen Inflammation of Keratinocytes in Psoriasis by Inhibiting EGR1-induced Transcription Activation of CHAC1.Current Molecular Medicine,,
MLA：
Li Yadi,et al."Caffeic Acid Reduces Ferroptosis to Dampen Inflammation of Keratinocytes in Psoriasis by Inhibiting EGR1-induced Transcription Activation of CHAC1".Current Molecular Medicine .(2024)

相关文献

[1]Targeted siRNA Therapy for Psoriasis: Translating Preclinical Potential into Clinical Treatments [2]Treatment of Moderate to Severe Plaque Psoriasis [3]Single-cell integrated transcriptomics reveals the role of keratinocytes in head and neck squamous cell carcinoma [4]组胺对皮肤角化上皮屏障功能的作用的研究 [5]The binding of extracellular cyclophilin A to ACE2 and CD147 triggers psoriasis-like inflammation [6]NOVEL EVALUATIONS OF PROTOCATECHUIC ACID TO TARGET IL-17A FOR PSORIASIS PROTECTION IN MICE [7]A Study of Guselkumab (TREMFYA) in Chinese Participants With Moderate to Severe Plaque Psoriasis [8]Klotho Protein Protects Human Keratinocytes from UVB-Induced Damage Possibly by reducing Expression and Nuclear Translocation of NF-kappa B [9]Tazarotene/Betamethasone Dipropionate Cream in Patients with Plaque Psoriasis: Results of a Prospective, Multicenter, Observational Study [10]Anionic surfactants and commercial detergents decrease tight junction barrier integrity in human keratinocytes

资源点击量：21166 今日访问量：0 总访问量：1219 更新日期：2025-01-01 建议使用谷歌、火狐浏览器 常见问题

版权所有©2020 首都医科大学附属北京同仁医院 技术支持：重庆聚合科技有限公司 地址：北京市东城区东交民巷1号(100730)