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Unmasking of molecular players: proteomic profiling of vitreous humor in pathologic myopia

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机构： [1]Department of Ophthalmology, Beijing Key Laboratory of Diagnosis and Therapy of Retinal and Choroid Diseases, Peking University People’s Hospital, Beijing, China [2]College of Optometry, Peking University People’s Hospital, Beijing, China [3]Eye Disease and Optometry Institute, Beijing, China [4]Peking University Health Science Center, No. 11 South Avenue of XiZhiMen, Xi Cheng District, 100044 Beijing, China

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关键词： Pathologic myopia (PM) Proteomics Chorioretinal cell apoptosis Scleral extracellular matrix (ECM) synthesis Scleral remodeling

摘要：

This study aimed to identify the differentially expressed proteins in the vitreous humor (VH) of eyes with and without pathologic myopia (PM), providing insights into the molecular pathogenesis.A cross-sectional, observational study was conducted. VH samples were collected from patients undergoing vitrectomy for idiopathic epiretinal membrane (ERM), macular hole (MH), or myopic retinoschisis (MRS). Label-free quantitative proteomic analysis identified differential protein expression, with validation using ELISA.The proteomic profiling revealed significantly higher expressions of tubulin alpha 1a (TUBA1A) and eukaryotic translation elongation factor 1 alpha 1 (EEF1A1) in PM groups (MH-PM, MRS-PM) compared to controls (MH, ERM). Conversely, xylosyltransferase 1 (XYLT1), versican core protein (VCAN), and testican-2 (SPOCK2) expressions were lower in PM. ELISA validation confirmed these findings.Our study provides novel insights into the molecular mechanisms of PM. The differentially expressed proteins EEF1A1, TUBA1A, XYLT1, VCAN, and SPOCK2 may play crucial roles in chorioretinal cell apoptosis, scleral extracellular matrix (ECM) synthesis, and scleral remodeling in PM. These proteins represent potential new targets for therapeutic intervention in PM, highlighting the importance of further investigations to elucidate their functions and underlying mechanisms in disease pathogenesis.© 2024. The Author(s).

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出版当年[2023]版：

大类 | 4 区医学

小类 | 3 区眼科学

最新[2025]版：

大类 | 3 区医学

小类 | 3 区眼科学

第一作者：

第一作者机构： [1]Department of Ophthalmology, Beijing Key Laboratory of Diagnosis and Therapy of Retinal and Choroid Diseases, Peking University People’s Hospital, Beijing, China [2]College of Optometry, Peking University People’s Hospital, Beijing, China [3]Eye Disease and Optometry Institute, Beijing, China [4]Peking University Health Science Center, No. 11 South Avenue of XiZhiMen, Xi Cheng District, 100044 Beijing, China

通讯作者：

通讯机构： [1]Department of Ophthalmology, Beijing Key Laboratory of Diagnosis and Therapy of Retinal and Choroid Diseases, Peking University People’s Hospital, Beijing, China [2]College of Optometry, Peking University People’s Hospital, Beijing, China [3]Eye Disease and Optometry Institute, Beijing, China [4]Peking University Health Science Center, No. 11 South Avenue of XiZhiMen, Xi Cheng District, 100044 Beijing, China

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Unmasking of molecular players: proteomic profiling of vitreous humor in pathologic myopia

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