OBJECTIVE: To evaluate the effects of Zuyangping ((sic) (sic)(sic), ZYP) formula on wound healing in diabetic rats, as well as the molecular mechanisms involved. METHODS: The main compounds in ZYP formula were identified by the Liquid chromatography-tandem mass spectrometry. Sprague-Dawley rats, injected with streptozotocin (STZ) to establish diabetes model, then, formed a defective skin trauma in the back, and each group was treated with corresponding drugs once a day. Granulation was taken from each time node for histological analysis. The Western blotting was used to measured protein expression of advanced glycation end products receptor (RAGE) and hypoxia-inducible factor1 alpha (HIF-1 alpha) axis-related proteins. The relative expression levels of inflammatory cytokines and growth factors were measured by the enzyme-linked immunosorbent assay method. RESULTS: The main ingredients were identified in the ZYP formula. Histological analysis showed that the ZYP formula could inhibit the expression of inflammation, promote angiogenesis and collagen deposition. In addition, the ZYP formula could regulate the expression of RAGE and HIF1-alpha axis-related proteins, thus promoting the wound healing in diabetic rats. CONCLUSION: The ZYP formula could accelerate wound healing in diabetic rats. (c) 2024 JTCM. All rights reserved.