机构：[1]Key Laboratory of Major Diseases in Children, Ministry of Education, Beijing Children’s Hospital, Capital Medical University, Beijing, China.首都医科大学附属北京儿童医院[2]Laboratory of Immunology, Beijing Pediatric Research Institute, Beijing Children’s Hospital, Capital Medical University, Beijing, China.首都医科大学附属北京儿童医院[3]Department of Otolaryngology, Head and Neck Surgery, Beijing Children’s Hospital, Capital Medical University, Beijing, China.首都医科大学附属北京儿童医院[4]Department of Otolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing, China.临床科室耳鼻咽喉-头颈外科首都医科大学附属北京同仁医院首都医科大学附属同仁医院[5]Beijing Key Laboratory for Pediatric Diseases of Otolaryngology, Head and Neck Surgery, Beijing Pediatric Research Institute, Beijing Children’s Hospital, Capital Medical University, Beijing, China.首都医科大学附属北京儿童医院
Whether humoral immunity plays a role in HPV type 6 or 11 virus-mediated Juvenile-onset Recurrent Respiratory Papillomatosis (JORRP) remains unknown. In the present study, serum total IgG level in 44 JORRP patients was significantly decreased compared with that in 40 healthy controls. Moreover, expanded CD3(-)CD19(+) B cells with down-regulation of CD23, CD40, HLA-DR and up-regulation of CD86 expression were found in the peripheral blood of JORRP patients. Flow cytometry analysis of B-cell compartment showed that the frequency of both CD19(+) CD27(hi) plasma cells and CD19(+) CD27(+) memory B cells were decreased in JORRP patients. Importantly, although the proportion of circulating CXCR5(+) PD1(hi) Tfh cells was not changed, the function of Tfh cells were greatly impaired with reduced ability of IL-21 secretion to promote B cell maturation. Association analysis by the Kaplan-Meier method revealed that IL-21 secreting Tfh cell was positively correlated to the CD27(+) B cell subset frequency, the serum IgG level and the frequency of recurrence in JORRP patients, but negatively correlated to the percentage of IgD(+) CD27(-) B cell. We concluded that a reduced IL-21 secretion by Tfh cells may limit B cell maturation and antibody production in JORRP patients and Tfh cell-derived IL-21 might be associated with JORRP outcome in clinic.
基金：
This study was supported by a National Natural Science Foundation of China (31470862), and partially supported
by a startup fund to JG from Beijing Children’s Hospital, a teaching hospital affiliated to Capital Medical University.
第一作者机构：[1]Key Laboratory of Major Diseases in Children, Ministry of Education, Beijing Children’s Hospital, Capital Medical University, Beijing, China.[2]Laboratory of Immunology, Beijing Pediatric Research Institute, Beijing Children’s Hospital, Capital Medical University, Beijing, China.
共同第一作者：
通讯作者：
通讯机构：[1]Key Laboratory of Major Diseases in Children, Ministry of Education, Beijing Children’s Hospital, Capital Medical University, Beijing, China.[2]Laboratory of Immunology, Beijing Pediatric Research Institute, Beijing Children’s Hospital, Capital Medical University, Beijing, China.[3]Department of Otolaryngology, Head and Neck Surgery, Beijing Children’s Hospital, Capital Medical University, Beijing, China.[5]Beijing Key Laboratory for Pediatric Diseases of Otolaryngology, Head and Neck Surgery, Beijing Pediatric Research Institute, Beijing Children’s Hospital, Capital Medical University, Beijing, China.
推荐引用方式(GB/T 7714)：
Wu Xunyao,Wang Guoliang,Chen Xi,et al.Impaired T Cell-dependent Humoral Immune Response Associated with Juvenile-onset Recurrent Respiratory Papillomatosis Progression[J].SCIENTIFIC REPORTS.2016,6:doi:10.1038/srep36378.
APA：
Wu, Xunyao,Wang, Guoliang,Chen, Xi,Zhang, Jie,Zhao, Jing...&Ni, Xin.(2016).Impaired T Cell-dependent Humoral Immune Response Associated with Juvenile-onset Recurrent Respiratory Papillomatosis Progression.SCIENTIFIC REPORTS,6,
MLA：
Wu, Xunyao,et al."Impaired T Cell-dependent Humoral Immune Response Associated with Juvenile-onset Recurrent Respiratory Papillomatosis Progression".SCIENTIFIC REPORTS 6.(2016)
