Background: Surface enhanced laser desorption and ionization time-of-flight mass spectrometry (SELDI-TOF-MS) analysis on serum samples was reported to be able to detect colorectal cancer (CRC) from normal or control patients. We carried out a validation study of a SELDI-TOF MS approach with IMAC surface sample processing to identify CRC. Methods: A retrospective cohort of 338 serum samples including 154 CRCs, 67 control cancers and 117 non-cancerous conditions was profiled using SELDI-TOF-MS. Results: No CRC "specific" classifier was found. However, a classifier consisting of two protein peaks separates cancer from non-cancerous conditions with high accuracy. Conclusion: In this study, the SELDI-TOF-MS-based protein expression profiling approach did not perform to identify CRC. However, this technique is promising in distinguishing patients with cancer from a non-cancerous population; it may be useful for monitoring recurrence of CRC after treatment.
基金：
Capital Development of Medical Research Fund [2005-1019]
第一作者机构：[1]Peking Univ, Key Lab Carcinogenesis & Translat Res, Minist Educ,Beijing Canc Hosp & Inst, Dept Colorectal Surg,Sch Oncol, Beijing 100871, Peoples R China[6]Huazhong Univ Sci & Technol, Hepat Surg Ctr, Tongji Hosp, Tongji Med Coll, Wuhan 430074, Peoples R China
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推荐引用方式(GB/T 7714)：
Wang Qi,Shen Jing,Li Zhen-fu,et al.Limitations in SELDI-TOF MS whole serum proteomic profiling with IMAC surface to specifically detect colorectal cancer[J].BMC CANCER.2009,9:doi:10.1186/1471-2407-9-287.
APA：
Wang, Qi,Shen, Jing,Li, Zhen-fu,Jie, Jian-zheng,Wang, Wen-yue...&Gu, Jin.(2009).Limitations in SELDI-TOF MS whole serum proteomic profiling with IMAC surface to specifically detect colorectal cancer.BMC CANCER,9,
MLA：
Wang, Qi,et al."Limitations in SELDI-TOF MS whole serum proteomic profiling with IMAC surface to specifically detect colorectal cancer".BMC CANCER 9.(2009)
