机构：[1]Department of Pediatrics, Nanjing Maternity and Child Health Care Hospital, Women’s Hospital of Nanjing Medical University, Nanjing 210029, China.[2]Department of Internal Medicine, Nantong Maternal and Child Health Care Hospital, Affiliated to Nantong University, Nantong, China.[3]Hongqiao International Institute of Medicine, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Background Obesity is a global epidemic disease that increases the risk of metabolic syndrome. However, therapeutic drugs for obesity are still scarce. In recent years, peptides have been identified as new biological regulators. RIFV (R-I-F-V-P-I-K-G-R-P-A-P), a novel active peptide from our peptide database. Methods We performed oil red O staining and triglyceride measurement to analyze the influence of RIFV on white preadipocytes differentiation. Then the effects of RIFV on cell proliferation, apoptosis and cell cycle were determined by using CCK-8 assay and flow cytometry. The mRNA and protein levels of adipogenesis-related genes were respectively detected by qRT-PCR and western blot. Rescue experiment was conducted to confirm whether RIFV could regulate adipocytes differentiation via targeting C/EBP-beta. Finally, the luciferase reporter gene assay was performed to verify the regulation of RIFV on C/EBP-beta gene. Results RIFV was revealed to inhibit the differentiation of human white adipocytes without affecting their proliferation. Additionally, RIFV could also suppress the differentiation of mouse primary white preadipocytes isolated from inguinal fat tissues. Furthermore, RIFV may have an inhibitory effect on adipogenesis by inhibiting the regulation of the adipogenic gene C/EBP-beta. Conclusions Our results indicated that RIFV may be a novel essential regulator of adipocyte differentiation and represents a therapeutic strategy for obesity and related complications.
基金：
This study was funded by grants from the National Natural Science
Foundation of China (Grant No. 81600665, 81770837, 81770866, 81700744,
81670773, and 81600687), the Jiangsu Provincial Medical Innovation Team
(CXTDA2017001), Jiangsu Provincial Medical Youth Talent (Grant No.
QNRC2016109 and QNRC2016108) and the Jiangsu Provincial Key Research
and Development Program (Grant No. BE2016619, BE2018616 and
BE2018614).
第一作者机构：[1]Department of Pediatrics, Nanjing Maternity and Child Health Care Hospital, Women’s Hospital of Nanjing Medical University, Nanjing 210029, China.[2]Department of Internal Medicine, Nantong Maternal and Child Health Care Hospital, Affiliated to Nantong University, Nantong, China.
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Zhang Wen,Shen Dan,Li Yun,et al.A novel peptide RIFV suppresses human adipocyte differentiation through the inhibition of C/EBP-beta expression[J].NUTRITION & METABOLISM.2019,16(1):doi:10.1186/s12986-019-0414-z.
APA：
Zhang, Wen,Shen, Dan,Li, Yun,Zhong, Hong,Wang, Xing...&Chen, Ling.(2019).A novel peptide RIFV suppresses human adipocyte differentiation through the inhibition of C/EBP-beta expression.NUTRITION & METABOLISM,16,(1)
MLA：
Zhang, Wen,et al."A novel peptide RIFV suppresses human adipocyte differentiation through the inhibition of C/EBP-beta expression".NUTRITION & METABOLISM 16..1(2019)
