Parkinson's disease (PD) is the second most common neurodegenerative disorder, characterized by dopaminergic neuron loss, inflammation and oxidative stress injury in the substantia nigra pars compacta (SNpc). Tripartite motif 10 (TRIM10) belongs to the TRIM family of proteins and has been implicated to play a role in in PD, although supporting evidence has yet to be established. 1-methyl-4-phenylpyridinium (MPP+), the metabolite of MPTP (Mitochondrial parkinsonian neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetra-hydropyridine), is often used to generate a cellular model of PD. In this study, we found that MPP + inhibited cell proliferation and induced TRIM10 expression. Knockdown of TRIM10 alleviated cell apoptosis and ROS generation induced by MPP+. Further, MPP + decreased the expression of dual specificity phosphatase 6 (DUSP6) and this effect was reversed by TRIM10 knockdown. Moreover, DUSP6 alleviated cell apoptosis and ROS generation induced by TRIM10. Of note, TRIM10 suppressed DUSP6 by promoting DUSP6 ubiquitination. In conclusion, silencing of TRIM10 reduced cell apoptosis and ROS levels in a cellular model of PD, suggesting a potential role of TRIM10 in PD treatment. (C) 2019 Published by Elsevier Inc.
基金：
Shanghai Tongren Hospital, Shanghai Jiaotong University School of Medicine [TRYJ(LC)201803]
第一作者机构：[1]Shanghai Jiao Tong Univ, Tongren Hosp, Sci Res Management Dept, Sch Med, Shanghai, Peoples R China
通讯作者：
通讯机构：[2]Shanghai Jiao Tong Univ, Tongren Hosp, Dept Neurol, Sch Med, 1111 XianXia Rd, Shanghai 200336, Peoples R China[*1]Department of Neurology, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, 1111 XianXia Road, Shanghai, 200336, China.[*2]Department of Neurology, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, 1111 XianXia Road, Shanghai, 200336, China.
推荐引用方式(GB/T 7714)：
Huang Qiong,Zhu Xiaoqi,Xu Min.Silencing of TRIM10 alleviates apoptosis in cellular model of Parkinson's disease[J].BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS.2019,518(3):451-458.doi:10.1016/j.bbrc.2019.08.041.
APA：
Huang, Qiong,Zhu, Xiaoqi&Xu, Min.(2019).Silencing of TRIM10 alleviates apoptosis in cellular model of Parkinson's disease.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,518,(3)
MLA：
Huang, Qiong,et al."Silencing of TRIM10 alleviates apoptosis in cellular model of Parkinson's disease".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 518..3(2019):451-458
