ObjectiveTo investigate the role of human papillomavirus (HPV) in laryngeal squamous cell carcinoma (LSCC) and analyse the relationship between HPV and the expression of cell cycle-related proteins. DesignThe study consisted of LSCC between 2005 and 2011 in Tongren Hospital. Clinical data such as age, sex, smoking/alcohol consumption, and TNM stage were collected. HPV DNA and cell cycle-related proteins were assessed in terms of clinical features. SettingSingle-centre study. ParticipantsA total of 332 LSCC patients were included in the study. Main outcome measuresThe presence of genotype-specific HPV DNA was evaluated using PCR-RDB in formalin-fixed paraffin-embedded tissues. All samples were also evaluated for p16(INK4A), p21(WAF1/CIP1), P53, Cyclin D1, and Ki67 immunohistochemical staining by tissue microarray. ResultsHPV DNA was detected in 45 of 332 (13.55%) patients with LSCC, with HPV-16 being the predominant genotype. The presence of HPV-16 DNA was significantly associated with basaloid squamous cell carcinoma and cystic lymph node metastasis (P<0.05). Of the 332 patients, 36 (10.84%) were scored as p16(INK4A) positivity and they were more likely to be female (P<0.05). Cyclin D1-positivity and p21(WAF1/CIP1)-positivity were observed in 60.24% (200/332) and 40.66% (135/332), respectively. In 114 cases (34.33%), LSCCs had moderate- to -strong p53 accumulation, which was correlated with TNM stage (P<0.05). HPV-16 DNA was correlated with p16(INK4A) and manifested a higher Ki-67 labelling index and p21(WAF1/CIP1) expression than HPV-16-negative tumours (P<0.05). No relationship was observed between Cyclin D1or P53 expression and HPV-16 infection (P>0.05). ConclusionHPV DNA was detected in 13.55% patients with LSCC, with HPV-16 being the predominant genotype and it was correlated with p16(INK4A) and manifested a higher Ki-67 labelling index and p21(WAF1/CIP1) expression than HPV-16-negative tumours.
基金：
Capital Health Research and Development of Special Foundation [2014-2-2053]
第一作者机构：[1]Capital Med Univ, Beijing Tongren Hosp, Dept Pathol, Beijing Key Lab Head & Neck Mol Diagnost Pathol, Beijing, Peoples R China
通讯作者：
通讯机构：[1]Capital Med Univ, Beijing Tongren Hosp, Dept Pathol, Beijing Key Lab Head & Neck Mol Diagnost Pathol, Beijing, Peoples R China[*1]Department of Pathology, Beijing Key Laboratory of Head and Neck Molecular Diagnostic Pathology, Beijing Tongren Hospital, Capital Medical University, Beijing, China
推荐引用方式(GB/T 7714)：
Cui Lifang,Qu Congling,Liu Honggang.Association study of cell cycle proteins and human papillomavirus in laryngeal cancer in Chinese population[J].CLINICAL OTOLARYNGOLOGY.2019,44(3):323-329.doi:10.1111/coa.13296.
APA：
Cui, Lifang,Qu, Congling&Liu, Honggang.(2019).Association study of cell cycle proteins and human papillomavirus in laryngeal cancer in Chinese population.CLINICAL OTOLARYNGOLOGY,44,(3)
MLA：
Cui, Lifang,et al."Association study of cell cycle proteins and human papillomavirus in laryngeal cancer in Chinese population".CLINICAL OTOLARYNGOLOGY 44..3(2019):323-329
