Purposes: To (i) evaluate various methods for preserving refractive lenticules (RLs) from myopic eyes following small-incision lenticule extraction (SMILE), in order to (ii) establish a sound, standard storage RL preservative for clinical uses. Methods: In this prospective study, we compared freshly excised post-SMILE RLs (control group) with post-SMILE RLs (experimental group). Experimental group RLs were preserved in one of several preservatives: glycerol, allochroic silicagel desiccant, or Optisol. Following preservation in one of these three media, samples were evaluated by light microscopy (LM), and transmission (TEM) and scanning (SEM) electron microscopy on days-1, -3, -7, and -14. Results: Changes in cellular morphology were observed at all time points. Compared with fresh control-group RLs, there were significant histological changes in RLs preserved in glycerol and allochroic silicagel, but not Optisol. Comparison of the three methods revealed Optisol to be the best, followed by allochroic silica gel desiccant, followed by glycerol. RLs preserved in Optisol maintained the highest degree of viability and integrity. And the RLs viability and collagen density decreased with prolongation of storage time all. Conclusions: Optisol is a midterm corneal storage medium, which can maintain post-SMILE corneal RLs for 14 days, is a feasible and effective method for tissue storage.
基金：
Beijing "215" Program for Training of High-level Talents in the Health System [2013-2-23]
第一作者机构：[1]Capital Med Univ, Beijing Tongren Hosp, Beijing Tongren Eye Ctr, Beijing Key Lab Ophthalmol & Visual Sci, 8 Nei Ave, Beijing 100730, Peoples R China[2]Second Peoples Hosp Yunnan Prov, Yunnan Eye Inst, Refract Surg Ctr, Kunming, Yunnan, Peoples R China
通讯作者：
通讯机构：[1]Capital Med Univ, Beijing Tongren Hosp, Beijing Tongren Eye Ctr, Beijing Key Lab Ophthalmol & Visual Sci, 8 Nei Ave, Beijing 100730, Peoples R China[*1]Beijing Key Laboratory of Ophthalmology and Visual Science, Beijing Tongren Eye Center, Beijing Tongren Hospital of Capital Medical University, No. 8. Nei Avenue, ChongWenMen in Dong-cheng District, Beijing, 100730, China
推荐引用方式(GB/T 7714)：
Liang Gang,Wang Li,Pan Zhiqiang,et al.Comparison of the Different Preservative Methods for Refractive Lenticules following SMILE[J].CURRENT EYE RESEARCH.2019,44(8):832-839.doi:10.1080/02713683.2019.1597890.
APA：
Liang, Gang,Wang, Li,Pan, Zhiqiang&Zhang, Fengju.(2019).Comparison of the Different Preservative Methods for Refractive Lenticules following SMILE.CURRENT EYE RESEARCH,44,(8)
MLA：
Liang, Gang,et al."Comparison of the Different Preservative Methods for Refractive Lenticules following SMILE".CURRENT EYE RESEARCH 44..8(2019):832-839
