We aimed to investigate the effect of venoarterial extracorporeal membrane oxygenation (VA-ECMO) on immune function of the spleen and reactive oxygen species (ROS) during post-resuscitation in a porcine model. After 8min of untreated ventricular fibrillation and 6min of basic life support, pigs were randomized into two groups: Group 1 received VA-ECMO and Group 2 received conventional cardiopulmonary resuscitation. After successful return of spontaneous circulation, the hemodynamic status was determined and blood samples were collected at 0, 1, 2, 4, and 6h. Surviving pigs were euthanized 6h after return of spontaneous circulation, their spleens were harvested and the T-cells were separated. Then, we investigated immune function parameters of the spleen and ROS levels. VA-ECMO increased the return of spontaneous circulation and 6h survival rate after return of spontaneous circulation. Compared with the conventional cardiopulmonary resuscitation group, the VA-ECMO group showed increased superoxide dismutase and decreased malondialdehyde and ROS levels. Furthermore, VA-ECMO was associated with a high rate of CD4+ and CD4+/CD8+, high levels of interleukin 2, interferon , and interferon /interleukin 4, as well as high proliferation of lymphocytes. The apoptotic rate of T-cells was lower in the VA-ECMO group than it was in the conventional cardiopulmonary resuscitation group. VA-ECMO increased immune function of spleen and decreased ROS levels during post-resuscitation. Further research is expected to illustrate whether the differences in immune responses are due to ROS or some other perfusion related effect on spleen.
基金：
National Natural Science Foundation of China[81372025]; Annual Special Cultivation and Development Project for the Technology Innovation Base of the Beijing Key Laboratory of Cardiopulmonary Cerebral Resuscitation [Z151100001615056]
第一作者机构：[1]Capital Med Univ, Beijing Chao Yang Hosp, Beijing Key Lab Cardiopulm Cerebral Resuscitat, Beijing, Peoples R China
通讯作者：
通讯机构：[1]Capital Med Univ, Beijing Chao Yang Hosp, Beijing Key Lab Cardiopulm Cerebral Resuscitat, Beijing, Peoples R China[*1]Capital Med Univ, Key Lab Cardiopulm Cerebral Resuscitat, 8 Workers Stadium South Rd, Beijing 100020, Peoples R China
推荐引用方式(GB/T 7714)：
Zhang Qiang,Liu Bo,Zhao Lianxing,et al.Venoarterial Extracorporeal Membrane Oxygenation Increased Immune Function of Spleen and Decreased Reactive Oxygen Species During Post-Resuscitation[J].ARTIFICIAL ORGANS.2019,43(4):377-385.doi:10.1111/aor.13367.
APA：
Zhang, Qiang,Liu, Bo,Zhao, Lianxing,Lian, Yong,Yuan, Xiaoli...&Li, Chunsheng.(2019).Venoarterial Extracorporeal Membrane Oxygenation Increased Immune Function of Spleen and Decreased Reactive Oxygen Species During Post-Resuscitation.ARTIFICIAL ORGANS,43,(4)
MLA：
Zhang, Qiang,et al."Venoarterial Extracorporeal Membrane Oxygenation Increased Immune Function of Spleen and Decreased Reactive Oxygen Species During Post-Resuscitation".ARTIFICIAL ORGANS 43..4(2019):377-385
