Objectives This work aims to reveal the roles and related mechanisms of RNA binding protein PUM2 in osteosarcoma progression. Materials and methods Transcriptome analysis based on RNA sequencing data, real-time quantitative PCR (RT-qPCR), and western blot analysis were used to detect the expression of RBPs and miRNAs in OS and normal adjacent tissues, and the correlation between them in OS tissues. RT-qPCR, western blot, cell viability, transwell migration, tumour spheres formation and in vivo tumour formation assays were used to examine the effects of RBP PUM2 on OS progression. Additionally, RNA immunoprecipitation (RIP) assay combined with RNA sequencing was performed to determine the binding site of RBP PUM2 on STARD13 3 ' UTR. Luciferase reporter and RIP assays were used to confirm the binding of miRNAs or PUM2 on STARD13 3 ' UTR. Results PUM2 and STARD13 expression was significantly decreased in OS tissues, and positively correlated. Overexpression of PUM2 or STARD13 3 ' UTR inhibited OS cells proliferation, migration, and stemness. Mechanistically, PUM2 competitively bound to STARD13 3 ' UTR with miR-590-3p and miR-9. The inhibition of PUM2 on OS cells progression was attenuated by STARD13 knockdown or related miRNAs overexpression. Conclusion PUM2 suppresses OS progression via partly and competitively binding to STARD13 3 ' UTR with miRNAs.
基金：
Shanghai Municipal Commission of Health
and Family Planning of Science and Research
Fund, Grant/Award Number: 20154Y0070;
National Natural Science Foundation of
China, Grant/Award Number: 81501897
第一作者机构：[1]TongRen Hospital, School of Medicine, Shanghai JiaoTong University, Shanghai, China
共同第一作者：
通讯作者：
通讯机构：[1]TongRen Hospital, School of Medicine, Shanghai JiaoTong University, Shanghai, China[*1]TongRen Hospital, School of Medicine, Shanghai JiaoTong University, 1111 Xianxia Road, Shanghai, China
推荐引用方式(GB/T 7714)：
Hu Ruixi,Zhu Xiaodong,Chen Chao,et al.RNA-binding protein PUM2 suppresses osteosarcoma progression via partly and competitively binding to STARD13 3 ' UTR with miRNAs[J].CELL PROLIFERATION.2018,51(6):doi:10.1111/cpr.12508.
APA：
Hu, Ruixi,Zhu, Xiaodong,Chen, Chao,Xu, Ruijun,Li, Yifan&Xu, Wei.(2018).RNA-binding protein PUM2 suppresses osteosarcoma progression via partly and competitively binding to STARD13 3 ' UTR with miRNAs.CELL PROLIFERATION,51,(6)
MLA：
Hu, Ruixi,et al."RNA-binding protein PUM2 suppresses osteosarcoma progression via partly and competitively binding to STARD13 3 ' UTR with miRNAs".CELL PROLIFERATION 51..6(2018)
生物学