机构：[1]Department of Otolaryngology, Daqing Oilfield General Hospital, Daqing City 163001, Heilongjiang Province, People’s Republic of China[2]Department of Otolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, 100730, Beijing, People’s Republic of China首都医科大学附属北京同仁医院临床科室耳鼻咽喉-头颈外科
Background: The aim of this study was to validate the antitumor function of EGFR-chimeric antigen T-cells (CART) targeted to FaDu cells, a hypopharyngeal squamous cell carcinoma cell line, and to provide a preclinical basis for the application of CART cell technology in hypopharyngeal squamous cell carcinoma. Methods: Detection of cytokine secretions of EGFR-CAR T and CART-controls in the presence of target cells and nontarget cells as an indicator of CART cell activation. Detection of the cytotoxic effects of EGFR-CAR T on specific tumors in the presence of target cells was evaluated by LDH release and CART cell proliferation. Results: The results showed that cytokine secretion increased significantly after EGFR-CAR T-cells were incubated with target cells, and EGFR-CAR T-cells has higher cytotoxic effect on target cells than the CART-control group. The target cell lysis rate was 52.66%. The proliferation of EGFR-CAR T-cells in the presence of target cells was not distinctly observed. Conclusion: In this study, we validated the antitumor function of EGFR-CAR T-cells targeted to the FaDu cell line and provided the foundation for application of the CART technique in the treatment of hypopharyngeal carcinoma.
第一作者机构：[1]Department of Otolaryngology, Daqing Oilfield General Hospital, Daqing City 163001, Heilongjiang Province, People’s Republic of China
共同第一作者：
通讯作者：
通讯机构：[2]Department of Otolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, 100730, Beijing, People’s Republic of China[*1]Department of Otolaryngology, Beijing Tongren Hospital, Capital Medical University, No 1 Dongjiaominxiang Street, Dongcheng District, Beijing, People’s Republic of China
推荐引用方式(GB/T 7714)：
Dong Yi-Han,Ding Yi-Ming,Guo Wei,et al.The functional verification of EGFR-CAR T-cells targeted to hypopharyngeal squamous cell carcinoma[J].ONCOTARGETS AND THERAPY.2018,11:7053-7059.doi:10.2147/OTT.S175516.
APA：
Dong, Yi-Han,Ding, Yi-Ming,Guo, Wei,Huang, Jun-Wei,Yang, Zheng...&Chen, Xiao-Hong.(2018).The functional verification of EGFR-CAR T-cells targeted to hypopharyngeal squamous cell carcinoma.ONCOTARGETS AND THERAPY,11,
MLA：
Dong, Yi-Han,et al."The functional verification of EGFR-CAR T-cells targeted to hypopharyngeal squamous cell carcinoma".ONCOTARGETS AND THERAPY 11.(2018):7053-7059
