PURPOSE: To characterize and quantify the total retinal vascular bed area (RVBA) and vascular density (VD) in normal eyes using stereographically corrected ultra-wide field (UWF) fluorescein angiography (FA). DESIGN: Cross-sectional study. METHODS: Fifty-nine eyes of 31 normal subjects with no evidence of ocular disease underwent FA using the Optos 200Tx (Optos plc, Dunfermline, United Kingdom). Central and peripheral-steered FA images were montaged and stereographically projected. The early-phase FA was used to extract the retinal vasculature as a binary mask. Two independent, masked reading center-certified graders delineated the peripheral edge of the total retinal area (TRA) on a middle-phase frame. The RVBA and TRA were computed automatically in mm(2) using software provided by the manufacturer. The VD was calculated by dividing RVBA by TRA. RESULTS: The mean RVBA was 42.3 +/- 14.8 mm(2) for the entire retina, with no difference between male and female subjects (P =.439) or between right and left eyes (P =.407). The mean VD was 4.3% +/- 1.4% for the entire retina, with no difference based on sex (P =.629) or laterality (P =.426). A negative correlation was observed between age and both RVBA and VD (RVBA: R = -0.6, P <.001; VD: R = -0.52, P <.001). Pairwise comparisons showed that subjects aged >65 years had smaller RVBA and VD than those younger than 50 years for the entire retina and in all quadrants. CONCLUSIONS: RVBA and VD can be extracted from UWF FA images using automated processing techniques and may provide a reliable alternative to measures such as nonperfusion area. (C) 2018 Elsevier Inc. All rights reserved.