机构：[1]Department of Otolaryngology-Head and Neck Surgery, The Second Affiliated Hospital of Anhui Medical University, Hefei 230061, China[2]Department of Otolaryngology-Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Key Laboratory of Otolaryngology Head and Neck Surgery, Beijing, China临床科室耳鼻咽喉-头颈外科首都医科大学附属北京同仁医院首都医科大学附属同仁医院[3]Department of Head and Neck Surgery, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA[4]Department of Otolaryngology-Head and Neck Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, 230022, China[5]University of Pittsburgh, 200 Lothrop Street, Pittsburgh, PA 15213, USA[6]Department of Epidemiology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA
Background: The purpose of this study was to elucidate IL-12R beta 2's roles as a tumor-associated immunological molecule, delineate the complex roles of IL-12R beta 2(+) tumor-infiltrating lymphocytes (TILs) and tumor cell IL-12R beta 2 expression in the tumor microenvironment, and determine the correlation of IL-12R beta 2(+) TILs and tumor cell IL-12R beta 2 expression with clinical prognosis. Methods: We assessed mRNA and protein levels in matched laryngeal cancer tissues (LTs) and adjacent normal mucous membrane tissues (ANMMTs) from 3 laryngeal cancer (LC) patients and ratios of IL-12R beta 2(+) TILs in matched LTs and ANMMTs from 61 LC patients. We used the Kaplan-Meier log-rank test and Cox regression hazard ratios to analyze survival. Results: Comparative proteomic and transcriptomic assays revealed that matched LTs and ANMMTs from the 3 patients had significantly different IL-12R beta 2 and IFN-gamma expression; the ratio of IL-12R beta 2(+) TILs decreased with lower degrees of tumor differentiation. Among all 61 LC patients, the IL-12R beta 2(+) TIL ratio in ANMMTs (38.5% +/- 22.8%) was significantly higher than that in LTs (29.7% +/- 19%; p < .001). Kaplan-Meier analysis revealed that patients with an IL-12R beta 2(+) TIL ratio >= 35% had significantly better survival than those with an IL12R beta 2(+) TIL ratio < 35% (log rank p = 0.041). Multivariable analysis showed a significant association between a high IL-12R beta 2(+) TIL ratio and overall survival (hazard ratio, 0.14; 95% confidence interval, 0.03-0.77). Conclusion: Tumor cell differentiation is associated with TILs' expression of IL-12R beta 2, and an IL-12R beta 2(+) TIL ratio >= 35%) indicates favorable prognosis in LC.
基金：
Anhui Provincial National Foundation [1408085MH157]; Key Science and Technology Project of Anhui Province [1501041147]
第一作者机构：[1]Department of Otolaryngology-Head and Neck Surgery, The Second Affiliated Hospital of Anhui Medical University, Hefei 230061, China[2]Department of Otolaryngology-Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Key Laboratory of Otolaryngology Head and Neck Surgery, Beijing, China
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推荐引用方式(GB/T 7714)：
Tao Ye,Gross Neil,Liu Yehai,et al.A high ratio of IL-12Rβ2-positive tumor-infiltrating lymphocytes indicates favorable prognosis in laryngeal cancer[J].ORAL ONCOLOGY.2017,74:148-156.doi:10.1016/j.oraloncology.2017.10.006.
APA：
Tao, Ye,Gross, Neil,Liu, Yehai,Zhang, Liyong,Li, Guojun...&Yang, Jianming.(2017).A high ratio of IL-12Rβ2-positive tumor-infiltrating lymphocytes indicates favorable prognosis in laryngeal cancer.ORAL ONCOLOGY,74,
MLA：
Tao, Ye,et al."A high ratio of IL-12Rβ2-positive tumor-infiltrating lymphocytes indicates favorable prognosis in laryngeal cancer".ORAL ONCOLOGY 74.(2017):148-156
医学