机构：[1]Doheny Eye Inst, 1355 San Pablo St, Los Angeles, CA 90033 USA[2]Univ Calif Los Angeles, David Geffen Sch Med, Dept Ophthalmol, Los Angeles, CA 90095 USA[3]Capital Med Univ, Beijing Tongren Hosp, Beijing Tongren Eye Ctr, Beijing Inst Ophthalmol, Beijing, Peoples R China首都医科大学附属北京同仁医院研究所眼科研究所[4]Univ Calif San Diego, Hamilton Glaucoma Ctr, San Diego, CA 92103 USA[5]Univ Calif San Diego, Shiley Eye Inst, San Diego, CA 92103 USA
Purpose: To evaluate the feasibility of safely performing aqueous angiography in intact eyes of living nonhuman primates (NHPs) for evaluating aqueous humor outflow and segmental patterns. Design: Cross-sectional, observational study. Subjects: Six nonhuman primates. Methods: Aqueous angiography was performed in 6 nonhuman primates. After anesthesia, an anterior chamber (AC) maintainer was placed through a temporal 1-mm side-port wound. Indocyanine green (ICG; 0.4%) or 2.5% fluorescein was introduced (individually or in sequence) into the eye with a gravity-driven constant-pressure system. Aqueous angiography images were obtained with a Spectralis HRA+OCT (Heidelberg Engineering GmbH, Heidelberg, Germany) suspended over the NHP eye using a custom-designed surgical boom arm. Concurrent anterior segment optical coherence tomography (OCT) was performed on distally angiographically positive and negative regions. Main Outcome Measures: Angiographic patterns described by location, time-course, choice of tracer, and anterior-segment OCT. Results: Aqueous angiography in the living NHP eye demonstrated mostly stable angiographic patterns. With multimodal imaging, angiographically positive signal co-localized with episcleral veins as identified by infrared imaging and intrascleral lumens, as demonstrated by anterior segment OCT. Sequential aqueous angiography in individual eyes with ICG followed by fluorescein showed similar angiographic patterns. A pulsatile nature of aqueous angiographic outflow was sometimes observed. Aqueous angiographic patterns could also dynamically change. In some instances, positive angiographic flow suddenly arose in regions previously without an angiographic signal. Alternatively, an angiographic signal could suddenly disappear from regions in which an angiographic signal was initially documented. Conclusions: Aqueous angiography in living NHPs demonstrated segmental and pulsatile patterns with a newly described ability to dynamically shift. These characteristics further the understanding of live aqueous humor outflow biology and may be useful in improving glaucoma surgeries aimed at trabecular meshwork bypass. (C) 2017 by the American Academy of Ophthalmology
基金：
National Institutes of Health, Bethesda, MD (grant nos.
K08EY024674 [A.S.H.]; American Glaucoma Society (AGS) Mentoring
for Physician Scientists Award 2014 [A.S.H.]; AGS Young Clinician Scientist
Award 2015 [A.S.H.]; Research to Prevent Blindness Career
Development Award 2016 [A.S.H.]; an unrestricted grant from Research to
Prevent Blindness (New York, NY); National Natural Science Foundation
of China (N.W.; 81271005 and 81300767), Beijing Municipal Administration
of Hospitals Clinical Medicine Development of Special Funding
Support (N.W.; ZYLX201501); Beijing Scholars Program (N.W.); and
China Health and Medical Development Foundation (N.W.).
第一作者机构：[1]Doheny Eye Inst, 1355 San Pablo St, Los Angeles, CA 90033 USA[2]Univ Calif Los Angeles, David Geffen Sch Med, Dept Ophthalmol, Los Angeles, CA 90095 USA[*1]Univ Calif Los Angeles, David Geffen Sch Med, Dept Ophthalmol, Doheny Eye Inst, 1355 San Pablo St, Los Angeles, CA 90033 USA
通讯作者：
通讯机构：[1]Doheny Eye Inst, 1355 San Pablo St, Los Angeles, CA 90033 USA[2]Univ Calif Los Angeles, David Geffen Sch Med, Dept Ophthalmol, Los Angeles, CA 90095 USA[3]Capital Med Univ, Beijing Tongren Hosp, Beijing Tongren Eye Ctr, Beijing Inst Ophthalmol, Beijing, Peoples R China[*1]Univ Calif Los Angeles, David Geffen Sch Med, Dept Ophthalmol, Doheny Eye Inst, 1355 San Pablo St, Los Angeles, CA 90033 USA[*2]Capital Med Univ, Beijing Tongren Hosp, Beijing Tongren Eye Ctr, Beijing, Peoples R China
推荐引用方式(GB/T 7714)：
Huang Alex S.,Li Meng,Yang Diya,et al.Aqueous Angiography in Living Nonhuman Primates Shows Segmental, Pulsatile, and Dynamic Angiographic Aqueous Humor Outflow[J].OPHTHALMOLOGY.2017,124(6):793-803.doi:10.1016/j.ophtha.2017.01.030.
APA：
Huang, Alex S.,Li, Meng,Yang, Diya,Wang, Huaizhou,Wang, Ningli&Weinreb, Robert N..(2017).Aqueous Angiography in Living Nonhuman Primates Shows Segmental, Pulsatile, and Dynamic Angiographic Aqueous Humor Outflow.OPHTHALMOLOGY,124,(6)
MLA：
Huang, Alex S.,et al."Aqueous Angiography in Living Nonhuman Primates Shows Segmental, Pulsatile, and Dynamic Angiographic Aqueous Humor Outflow".OPHTHALMOLOGY 124..6(2017):793-803
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