机构：[1]Department of Clinical Laboratory, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China[2]Shanghai Research Institute of Stomatology, Shanghai 200011, China[3]Hongqiao International Institute of Medicine, Shanghai Tongren Hospital/Faculty of Basic Medicine, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China[4]Key Laboratory of Cell Differentiation and Apoptosis of the Chinese Ministry of Education, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
Ubiquitin-specific protease 14, a deubiquitinating enzyme, has been implicated in the tumorigenesis and progression of several cancers, but its role in oral squamous cell carcinoma remains to be elucidated. The aim of this study was to explore the expression pattern and roles of Ubiquitin-specific protease 14 in the occurrence and development of oral squamous cell carcinoma. Interestingly, Ubiquitin-specific protease 14 was overexpressed in oral cancer tissues and cell lines at both mRNA and protein levels. b-AP15, a specific inhibitor of Ubiquitin-specific protease 14, significantly inhibited the growth of cancer cells and increased cell apoptosis in a dose-dependent manner. Moreover, knockdown of Ubiquitin-specific protease 14 by shRNA significantly inhibited the proliferation and migration of cancer cells in vitro. Finally, using a xenograft mouse model of oral squamous cell carcinoma, knockdown of Ubiquitin-specific protease 14 markedly inhibited tumor growth and triggered the cancer cell apoptosis in vivo, supporting previous results. In conclusion, for the first time we have demonstrated the expression pattern of Ubiquitin-specific protease 14 in oral squamous cell carcinoma and verified a relationship with tumor growth and metastasis. These results may highlight new therapeutic strategies for tumor treatment, application of Ubiquitin-specific protease 14 selective inhibitor, such as b-AP15, or knockdown by shRNA. Collectively, Ubiquitin-specific protease 14 could be a potential therapeutic target for oral squamous cell carcinoma patients. (C) 2016 Elsevier Ltd. All rights reserved.
基金：
National Basic
Research Program of China (973 Program) (NO.2015CB910403),
National Natural Science Foundation of China (81101642,
81272886, 81570118), Science and Technology Committee of
Shanghai (15401901800), Innovation Program of Shanghai Municipal Education Commission (13YZ028), Shanghai bureau of health
Fund (2012173).
第一作者机构：[1]Department of Clinical Laboratory, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Clinical Laboratory, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China[3]Hongqiao International Institute of Medicine, Shanghai Tongren Hospital/Faculty of Basic Medicine, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China[4]Key Laboratory of Cell Differentiation and Apoptosis of the Chinese Ministry of Education, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China[*1]Department of Clinical Laboratory, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China[*2]Hongqiao International Institute of Medicine, Shanghai Tongren Hospital/Faculty of Basic Medicine, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China[*3]Key Laboratory of Cell Differentiation and Apoptosis of the Chinese Ministry of Education, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
推荐引用方式(GB/T 7714)：
Chen Xiangyun,Wu Jingjing,Chen Yitian,et al.Ubiquitin-specific protease 14 regulates cell proliferation and apoptosis in oral squamous cell carcinoma[J].INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY.2016,79:350-359.doi:10.1016/j.biocel.2016.08.038.
APA：
Chen, Xiangyun,Wu, Jingjing,Chen, Yitian,Ye, Dongxia,Lei, Hu...&Gu, Wenli.(2016).Ubiquitin-specific protease 14 regulates cell proliferation and apoptosis in oral squamous cell carcinoma.INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY,79,
MLA：
Chen, Xiangyun,et al."Ubiquitin-specific protease 14 regulates cell proliferation and apoptosis in oral squamous cell carcinoma".INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY 79.(2016):350-359
