机构：[1]Department of Otolaryngology Head and Neck Surgery and Department of Allergy, Beijing TongRen Hospital, Capital Medical University, Beijing 100730, China临床科室耳鼻咽喉-头颈外科变态反应科首都医科大学附属北京同仁医院首都医科大学附属同仁医院[2]Beijing Key Laboratory of Nasal diseases, Beijing Institute of Otolaryngology, Beijing 100005, China研究所耳鼻咽喉科研究所首都医科大学附属北京同仁医院首都医科大学附属同仁医院[3]Upper Airways Research Laboratory, Department of Oto-Rhino-Laryngology, Ghent University Hospital, De Pintelaan 185, 9000 Ghent, Belgium[4]Department of Otorhinolaryngology Head and Neck Surgery, the First Affiliated Hospital, Harbin Medical University, Harbin 150001, Heilongjiang Province, China
Purpose: Group 2 innate lymphoid cells (ILC2s) are a novel population of lineage-negative cells that induce innate type 2 responses by producing the critical Th2-type cytokines IL-5 and IL-13 in response to IL-25 and IL-33 stimulation. ILC2s accumulation in the peripheral blood of patients with allergic rhinitis (AR) is controversial; the precise role of ILC2s in the immunopathogenesis of AR is still not clear. We investigated the role of ILC2s in phenotypic AR sensitized to distinct allergens. Methods: Flow cytometric analysis of the peripheral blood of 7 healthy controls (HCs), 9 patients monosensitized to house dust mite (HDM), and 8 patients monosensitized to mugwort was performed to quantify ILC2s frequency. Peripheral blood mononuclear cells (PBMCs) were isolated from HDM-AR and mugwort-AR patients, and Lineage(-) and Lineage(+) cells were separated using a fluorescence-activated cell sorter (FACS). IL-5 and IL-13 levels in the supernatants of PBMCs, and Lineage and Lineage+ cells stimulated with IL-25 and/or IL-33 combined with IL-2 in vitro were assessed using the Milliplex magnetic bead kit. Results: The percentage of ILC2s was significantly elevated in HDM-AR patients compared to mugwort-AR patients and HCs, while no significant difference was found between mugwort-AR patients and HCs. IL-33 +/- IL-25 plus IL-2 induced a significantly greater release of IL-5 and IL-13 in the PBMCs of HDM-AR patients compared to PBMCs of mugwort-AR patients. IL-25 plus IL-2 also induced a significantly greater release of IL-13 in the PBMCs of HDM-AR patients compared to PBMCs of mugwort-AR patients. Stimulation with IL-33 and/or IL-25 combined with IL-2 also induced a significantly greater IL-5 and IL-13 release from Lineage(-) cells compared to Lineage(+) cells. Conclusions: AR patients sensitized to HDM or mugwort allergen have distinct phenotypic and functional profiles in ILC2s frequencies. ILC2s mediate major type 2 immunity in the development of HDM-AR and may be a potential therapeutic target.
基金：
program for Changjiang scholars and innovative research teamProgram for Changjiang Scholars & Innovative Research Team in University (PCSIRT) [IRT13082]; 12th five-year science and technology support project [2014BAI07B04]; national natural science fund for the major international joint research program [81420108009]; national natural science foundation of ChinaNational Natural Science Foundation of China (NSFC) [81100704, 81441029, 81441031]; Beijing natural science foundationBeijing Natural Science Foundation [7131006]; Beijing municipal administration of hospitals clinical medicine development of special funding support [ZYLX201310]; capital health research and development of special [2011-1017-06]; specialized research fund for the doctoral program of higher education of ChinaSpecialized Research Fund for the Doctoral Program of Higher Education (SRFDP) [20111107120004]; special fund of sanitation elite reconstruction of Beijing [2009-2-007]; Beijing health bureau program for high-level talents [2009-2-007, 2011-3-039, 2011-3-043, 2014-3-018]; Beijing Municipal Administration of Hospitals' Mission Plan [SML20150203]
第一作者机构：[1]Department of Otolaryngology Head and Neck Surgery and Department of Allergy, Beijing TongRen Hospital, Capital Medical University, Beijing 100730, China[2]Beijing Key Laboratory of Nasal diseases, Beijing Institute of Otolaryngology, Beijing 100005, China[4]Department of Otorhinolaryngology Head and Neck Surgery, the First Affiliated Hospital, Harbin Medical University, Harbin 150001, Heilongjiang Province, China
通讯作者：
通讯机构：[1]Department of Otolaryngology Head and Neck Surgery and Department of Allergy, Beijing TongRen Hospital, Capital Medical University, Beijing 100730, China[2]Beijing Key Laboratory of Nasal diseases, Beijing Institute of Otolaryngology, Beijing 100005, China[*1]Beijing Institute of Otolaryngology, No. 17, HouGouHuTong, DongCheng District, Beijing 100005, China.
推荐引用方式(GB/T 7714)：
Dachuan Fan,Xiangdong Wang,Min Wang,et al.Allergen-Dependent Differences in ILC2s Frequencies in Patients With Allergic Rhinitis[J].ALLERGY ASTHMA & IMMUNOLOGY RESEARCH.2016,8(3):216-222.doi:10.4168/aair.2016.8.3.216.
APA：
Dachuan Fan,Xiangdong Wang,Min Wang,Yang Wang,Liang Zhang...&Luo Zhang.(2016).Allergen-Dependent Differences in ILC2s Frequencies in Patients With Allergic Rhinitis.ALLERGY ASTHMA & IMMUNOLOGY RESEARCH,8,(3)
MLA：
Dachuan Fan,et al."Allergen-Dependent Differences in ILC2s Frequencies in Patients With Allergic Rhinitis".ALLERGY ASTHMA & IMMUNOLOGY RESEARCH 8..3(2016):216-222
