Major urinary proteins (MUPs) are the most abundant protein species in mouse urine, accounting for more than 90% of total protein content. Twenty-one Mup genes and 21 pseudogenes are clustered in a region of around 2 megabase pairs (Mbp) on chromosome 4. A Mup-knockout mouse model would greatly facilitate researches in the field of proteomic analysis of mouse urine. Here, we report the successful knockout of the Mup gene cluster of 2.2 Mbp using the CRISPR/Cas9 system. Homozygous Mup-knockout mice survived to adulthood and exhibited no obvious defects. The patterns of the proteomes of non-MUP urinary proteins in homozygous Mup-knockout mice were similar to those of wild-type mice judged by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The sensitivity of enzyme-linked immunosorbent assay to detect non-MUP urinary protein was significantly enhanced in Mup-knockout mice. In short, we have developed a Mup-knockout mouse model. This mouse model will be useful for the research of urinary biomarker testing that may have relevance for humans.
基金：
Major State Basic Research Development Program of ChinaNational Basic Research Program of China [2011CB812401, 2012CB837403]
第一作者机构：[1]Beijing Normal Univ, Coll Life Sci, Beijing 100875, Peoples R China[2]Natl Inst Biol Sci, Beijing 102206, Peoples R China
共同第一作者：
通讯作者：
通讯机构：[2]Natl Inst Biol Sci, Beijing 102206, Peoples R China[5]Natl Inst Biol Sci, Collaborat Innovat Ctr Canc Med, Beijing 102206, Peoples R China
推荐引用方式(GB/T 7714)：
Yang Haixia,Zhang Wei,Lu Shan,et al.Mup-knockout mice generated through CRISPR/Cas9-mediated deletion for use in urinary protein analysis[J].ACTA BIOCHIMICA ET BIOPHYSICA SINICA.2016,48(5):468-473.doi:10.1093/abbs/gmw003.
APA：
Yang, Haixia,Zhang, Wei,Lu, Shan,Lu, Guangqing,Zhang, Hongjuan...&Chen, Liang.(2016).Mup-knockout mice generated through CRISPR/Cas9-mediated deletion for use in urinary protein analysis.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,48,(5)
MLA：
Yang, Haixia,et al."Mup-knockout mice generated through CRISPR/Cas9-mediated deletion for use in urinary protein analysis".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 48..5(2016):468-473
