Objective: Perfluorooctanoic acid (PFOA) is widely used in consumer products and detected in human serum. Our study meant to elucidate the uncovered molecular mechanisms underlying the PFOA induced colorectal cancer cell DLD-1 invasion and matrix metalloproteinases (MMP) expression. Methods and results: Trans-well filter assay appeared that PFOA treatment stimulated DLD-1 cells invasion significantly. Meanwhile, the results of luciferase reporter, quantitative real-time PCR, western blotting, and gelatin zymography showed that PFOA induced MMP-2/-9 expression and enzyme activation levels consistently (P < 0.05 each). Subsequently, western blotting and immunofluorescence assay demonstrated that PFOA could enhance nuclear factor kappaB (NF-kappa B) activity by stimulating NF-kappa B translocation into nuclear in DLD-1 cells. Furthermore, JSH-23, a well-known NF-kappa B inhibitor, could reverse the PFOA induced colorectal cancer cell invasion and MMP-2/-9 expression. Conclusions: Our study confirmed that PFOA could induce colorectal cancer cell DLD-1 invasive ability and MMP-2/-9 expression through activating NF-kappa B, which deserves more concerns on environmental pollutant-resulted public health risk.
基金：
National Natural Science Foundation of China
(81302304) and Shanghai Municipal Health
Bureau Key Disciplines Grant (ZK2012A05).
第一作者机构：[2]Nanjing Med Univ, Affiliated Hosp 1, Dept Pathol, Nanjing 210029, Jiangsu, Peoples R China
共同第一作者：
通讯作者：
通讯机构：[1]Shanghai Jiao Tong Univ, Sch Med, Shanghai Tongren Hosp, Digest Endoscopy Ctr, Shanghai 200336, Peoples R China[*1]Digestive Endoscopy Center, Shanghai Tongren Hospital of Shanghai Jiao Tong University School of Medicine, Shanghai 200336, P. R. China
推荐引用方式(GB/T 7714)：
Miao Chen,Ma Jun,Zhang Yajie,et al.Perfluorooctanoic acid enhances colorectal cancer DLD-1 cells invasiveness through activating NF-kappa B mediated matrix metalloproteinase-2/-9 expression[J].INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY.2015,8(9):10512-10522.
APA：
Miao, Chen,Ma, Jun,Zhang, Yajie,Chu, Yimin,Li, Ji...&Peng, Haixia.(2015).Perfluorooctanoic acid enhances colorectal cancer DLD-1 cells invasiveness through activating NF-kappa B mediated matrix metalloproteinase-2/-9 expression.INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY,8,(9)
MLA：
Miao, Chen,et al."Perfluorooctanoic acid enhances colorectal cancer DLD-1 cells invasiveness through activating NF-kappa B mediated matrix metalloproteinase-2/-9 expression".INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY 8..9(2015):10512-10522
