Background: The pattern of epochal evolution of NoV is ongoing, while novel GII.4 variants emerge and cause new pandemics. Since, the emergence in March 2012, Sydney_2012 had replaced GII.4-2009 as the primary NoV strain in most countries in the northern hemisphere by November 2012. Objectives: To determine the genotype distribution around the emergence of Sydney_2012 and to investigate the underlying evolution mechanisms of the contemporary GII.4 strains. Study design: From January 2012 to December 2013, molecular epidemiology of norovirus in 846 adults (>= 16 years) in Shanghai were conducted. The VP1 proteins of the contemporary GII.4 strains (Den_Haag_2006b, New_Orleans_2009 and Sydney_2012) were expressed in vitro and purified. Receptor binding patterns of these three epidemic strains were determined through histo-blood group antigen (HBGA) binding assays. Convalescent serum from patients infected with GII.4 epidemic strains were employed to investigate the role of antigenic drift in the persistence of GII.4 epidemic strains through receptor-binding blockade assays. Results: Epidemiological studies revealed that Sydeny_2012 has completely replaced Den_Haag_2006b and New_Orleans_2009 and has been the dominant circulating strain in Shanghai since its emergence in October 2012. Interestingly, Den_Haag_2006b and New_Orleans_2009 have been co-circulating in Shanghai before the emergence of Sydeny_2012. The contemporary GII.4 epidemic norovirus strains displayed commonly high tropism to the histo-blood group antigen receptors, whereas Sydeny_2012 was antigenically different from Den_Haag_2006b and New_Orleans_2009. Conclusions: Antigenic drift, rather than receptor switch, played a key role in the emergence and spreading of Sydney_2012. The contemporary GII.4 strains were evolving via epochal evolution without altered ligand binding profiles. C) 2015 Elsevier BAT. All rights reserved.
基金：
grant no. 81470829 from National
Nature Science Foundation of China, grant no. 2012ZX10004-211
from the Ministry of Science and Technology of the People’s
Republic of China, grant no. 134119a9000 from the Science and
Technology Commission of Shanghai Municipality and grant no.
0134151 from Shanghai Municipal Commission of Health and Family Planning.
第一作者机构：[1]Tongji Univ, Dept Lab Med, Shanghai Matern & Infant Hosp 1, Shanghai 200092, Peoples R China[3]Fudan Univ, Dept Clin Lab, Shanghai Publ Hlth Clin Ctr, Shanghai 200433, Peoples R China[*1]Department of Laboratory Medicine, Shanghai First Maternity and Infant Hospital, Tongji University, No. 2699 West Gaoke Road, Pudong New Area, Shanghai, China.
共同第一作者：
通讯作者：
通讯机构：[1]Tongji Univ, Dept Lab Med, Shanghai Matern & Infant Hosp 1, Shanghai 200092, Peoples R China[3]Fudan Univ, Dept Clin Lab, Shanghai Publ Hlth Clin Ctr, Shanghai 200433, Peoples R China[4]Fudan Univ, Dept Pathogen Diag & Biosafety, Shanghai Publ Hlth Clin Ctr, Shanghai 200433, Peoples R China[*1]Department of Laboratory Medicine, Shanghai First Maternity and Infant Hospital, Tongji University, No. 2699 West Gaoke Road, Pudong New Area, Shanghai, China.[*2]Department of Pathogen Diagnosis and Biosafety, Shanghai Public Health Clinical Center, Fudan University, No. 2901 Caolang Road, Shanghai 201508, Jinshan District, China
推荐引用方式(GB/T 7714)：
Zhang Jun,Shen Zhen,Zhu Zhaoqin,et al.Genotype distribution of norovirus around the emergence of Sydney_2012 and the antigenic drift of contemporary GII.4 epidemic strains[J].JOURNAL OF CLINICAL VIROLOGY.2015,72:95-101.doi:10.1016/j.jcv.2015.09.009.
APA：
Zhang, Jun,Shen, Zhen,Zhu, Zhaoqin,Zhang, Wanju,Chen, Huifen...&Yuan, Zhenghong.(2015).Genotype distribution of norovirus around the emergence of Sydney_2012 and the antigenic drift of contemporary GII.4 epidemic strains.JOURNAL OF CLINICAL VIROLOGY,72,
MLA：
Zhang, Jun,et al."Genotype distribution of norovirus around the emergence of Sydney_2012 and the antigenic drift of contemporary GII.4 epidemic strains".JOURNAL OF CLINICAL VIROLOGY 72.(2015):95-101
