机构：[1]Hongqiao International Institute of Medicine, Shanghai Tongren Hospital and Faculty of Basic Medicine[2]Department of Pathophysiology, Shanghai Jiaotong University School of Medicine, (Room 1006, Building 2, No 280, South Chongqing Road), Shanghai 200025, People’s Republic of China
Kallistatin is a serine protease inhibitor (serpin) which specifically inhibits human tissue kallikrein; however, its inhibitory activity is inhibited by heparin. In order to elucidate the underlying mechanism, recombinant human kallistatin was prepared in Escherichia coli and the protein was crystallized by the sitting-drop vapour-diffusion method. X-ray diffraction data were collected to 1.9 angstrom resolution. The crystals were found to belong to space group P6(1), with unit-cell parameters a = 113.51, b = 113.51, c = 76.17 angstrom. Initial analysis indicated that the crystallized kallistatin was in a relaxed conformation, with its reactive-centre loop inserted in the central beta-sheet.
基金：
NSFCNational Natural Science Foundation of China (NSFC) [31170724, 31370727]
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2014]版：
无
最新[2023]版：
大类|4 区生物学
小类|4 区生化研究方法4 区生化与分子生物学4 区生物物理4 区晶体学
JCR分区：
出版当年[2013]版：
Q3CRYSTALLOGRAPHYQ4BIOPHYSICSQ4BIOCHEMISTRY & MOLECULAR BIOLOGYQ4BIOCHEMICAL RESEARCH METHODS
最新[2023]版：
Q3CRYSTALLOGRAPHYQ4BIOCHEMICAL RESEARCH METHODSQ4BIOCHEMISTRY & MOLECULAR BIOLOGYQ4BIOPHYSICS
第一作者机构：[1]Hongqiao International Institute of Medicine, Shanghai Tongren Hospital and Faculty of Basic Medicine[2]Department of Pathophysiology, Shanghai Jiaotong University School of Medicine, (Room 1006, Building 2, No 280, South Chongqing Road), Shanghai 200025, People’s Republic of China
通讯作者：
通讯机构：[1]Hongqiao International Institute of Medicine, Shanghai Tongren Hospital and Faculty of Basic Medicine[2]Department of Pathophysiology, Shanghai Jiaotong University School of Medicine, (Room 1006, Building 2, No 280, South Chongqing Road), Shanghai 200025, People’s Republic of China
推荐引用方式(GB/T 7714)：
Lin Fang,Zhou Aiwu,Wei Zhenquan.Crystallization and crystallographic studies of kallistatin[J].ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS.2015,71:1135-1138.doi:10.1107/S2053230X15012893.
APA：
Lin, Fang,Zhou, Aiwu&Wei, Zhenquan.(2015).Crystallization and crystallographic studies of kallistatin.ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS,71,
MLA：
Lin, Fang,et al."Crystallization and crystallographic studies of kallistatin".ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS 71.(2015):1135-1138
