机构：[1]Beijing Inst Basic Med Sci, Beijing 100850, Peoples R China[2]Acad Mil Med Sci, State Key Lab Toxicol & Med Countermeasures, Beijing 100850, Peoples R China[3]Acad Mil Med Sci, Lab Toxicant Anal, Inst Pharmacol & Toxicol, Beijing 100850, Peoples R China[4]Wuhan Univ, Wuhan City Hosp 3, Deparment Orthoped, Wuhan 430060, Peoples R China[5]Wuhan Univ, Tongren Hosp, Wuhan 430060, Peoples R China[6]Beijing Inst Lab Blood Borne Virus, Beijing 100850, Peoples R China
In this study, we further investigated a previously developed aptamer targeting ROS 17/2.8 (rat osteosarcoma) cells. We found that this C6-8 aptamer specifically binds to heterogeneous nuclear ribonucleoprotein (hnRNP) A2/B1 and that it specifically labeled multiple tumor-cell lines as effectively as hnRNP A2/B1 monoclonal antibodies. When conjugated with fluorescent carbon nanodots (CDots) it could freely enter multiple living tumor cell lines (HepG2, MCF-7, H1299, and HeLa), whose growth it inhibited by targeting hnRNP A2/B1. Similar inhibitory effects were observed when the GFP-HepG2 hepatocarcinoma cells treated with C6-8-conjugated CDots were implanted in nude mice. Our work provides a new aptamer for targeting/labeling multiple tumor cell types, and its nanoparticle conjugates bring further advantages that increase its potential for use in cancer diagnosis and therapy. (C) 2015 Elsevier Ltd. All rights reserved.
基金：
Chinese National High-Tech
Research and Development Program (2012AA022501), the National Natural Science Foundation of China (21175152, 81001262),
National Science and Technology Major Project of the Ministry of
Science and Technology of China (2012ZX09301003-001-010) and
the Beijing Municipal National Science Foundation (7132159).
第一作者机构：[1]Beijing Inst Basic Med Sci, Beijing 100850, Peoples R China
共同第一作者：
通讯作者：
通讯机构：[2]Acad Mil Med Sci, State Key Lab Toxicol & Med Countermeasures, Beijing 100850, Peoples R China[3]Acad Mil Med Sci, Lab Toxicant Anal, Inst Pharmacol & Toxicol, Beijing 100850, Peoples R China
推荐引用方式(GB/T 7714)：
Li Hui,Guo Lei,Huang Aixue,et al.Nanoparticle-conjugated aptamer targeting hnRNP A2/B1 can recognize multiple tumor cells and inhibit their proliferation[J].BIOMATERIALS.2015,63:168-176.doi:10.1016/j.biomaterials.2015.06.013.
APA：
Li, Hui,Guo, Lei,Huang, Aixue,Xu, Hua,Liu, Xuemei...&Shao, Ningsheng.(2015).Nanoparticle-conjugated aptamer targeting hnRNP A2/B1 can recognize multiple tumor cells and inhibit their proliferation.BIOMATERIALS,63,
MLA：
Li, Hui,et al."Nanoparticle-conjugated aptamer targeting hnRNP A2/B1 can recognize multiple tumor cells and inhibit their proliferation".BIOMATERIALS 63.(2015):168-176
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