BDNF Increases Survival and Neuronal Differentiation of Human Neural Precursor Cells Cotransplanted with a Nanofiber Gel to the Auditory Nerve in a Rat Model of Neuronal Damage
机构:[1]Center for Hearing and Communication Research, Karolinska University Hospital, 171 76 Stockholm, Sweden[2]Department of Clinical Sciences, Intervention and Technology (CLINTEC), Section of Otorhinolaryngology,Karolinska Institutet, Karolinska University Hospital, 171 76 Stockholm, Sweden[3]Department of Otolaryngology, Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University,Beijing 100730, China临床科室耳鼻咽喉-头颈外科首都医科大学附属北京同仁医院首都医科大学附属同仁医院[4]Department of Ophthalmology, Institution of Clinical Sciences in Lund, Lund University, 221 84 Lund, Sweden[5]Departments of Chemistry, Materials Science and Engineering, and Medicine and Institute for Bionanotechnology in Medicine,Northwestern University, Chicago, IL 60208, USA[6]Division of Otorhinolaryngology, Linkoping University Hospital, 581 85 Link ¨ oping, Sweden ¨[7]Division of Otorhinolaryngology, Department of Clinical and Experimental Medicine,University of Linkoping, 581 85 Link ¨ oping, Sweden
Objectives. To study possible nerve regeneration of a damaged auditory nerve by the use of stem cell transplantation. Methods. We transplanted HNPCs to the rat AN trunk by the internal auditory meatus (IAM). Furthermore, we studied if addition of BDNF affects survival and phenotypic differentiation of the grafted HNPCs. A bioactive nanofiber gel (PA gel), in selected groups mixed with BDNF, was applied close to the implanted cells. Before transplantation, all rats had been deafened by a round window niche application of beta-bungarotoxin. This neurotoxin causes a selective toxic destruction of the AN while keeping the hair cells intact. Results. Overall, HNPCs survived well for up to six weeks in all groups. However, transplants receiving the BDNF-containing PA gel demonstrated significantly higher numbers of HNPCs and neuronal differentiation. At six weeks, a majority of the HNPCs had migrated into the brain stem and differentiated. Differentiated human cells as well as neurites were observed in the vicinity of the cochlear nucleus. Conclusion. Our results indicate that human neural precursor cells (HNPC) integration with host tissue benefits from additional brain derived neurotrophic factor (BDNF) treatment and that these cells appear to be good candidates for further regenerative studies on the auditory nerve (AN).
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Q3BIOTECHNOLOGY & APPLIED MICROBIOLOGYQ3MEDICINE, RESEARCH & EXPERIMENTAL
第一作者机构:[1]Center for Hearing and Communication Research, Karolinska University Hospital, 171 76 Stockholm, Sweden[2]Department of Clinical Sciences, Intervention and Technology (CLINTEC), Section of Otorhinolaryngology,Karolinska Institutet, Karolinska University Hospital, 171 76 Stockholm, Sweden[3]Department of Otolaryngology, Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University,Beijing 100730, China
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通讯机构:[1]Center for Hearing and Communication Research, Karolinska University Hospital, 171 76 Stockholm, Sweden[2]Department of Clinical Sciences, Intervention and Technology (CLINTEC), Section of Otorhinolaryngology,Karolinska Institutet, Karolinska University Hospital, 171 76 Stockholm, Sweden
推荐引用方式(GB/T 7714):
Jiao Yu,Palmgren Bjorn,Novozhilova Ekaterina,et al.BDNF Increases Survival and Neuronal Differentiation of Human Neural Precursor Cells Cotransplanted with a Nanofiber Gel to the Auditory Nerve in a Rat Model of Neuronal Damage[J].BIOMED RESEARCH INTERNATIONAL.2014,2014:doi:10.1155/2014/356415.
APA:
Jiao, Yu,Palmgren, Bjorn,Novozhilova, Ekaterina,Johansson, Ulrica Englund,Spieles-Engemann, Anne L....&Olivius, Petri.(2014).BDNF Increases Survival and Neuronal Differentiation of Human Neural Precursor Cells Cotransplanted with a Nanofiber Gel to the Auditory Nerve in a Rat Model of Neuronal Damage.BIOMED RESEARCH INTERNATIONAL,2014,
MLA:
Jiao, Yu,et al."BDNF Increases Survival and Neuronal Differentiation of Human Neural Precursor Cells Cotransplanted with a Nanofiber Gel to the Auditory Nerve in a Rat Model of Neuronal Damage".BIOMED RESEARCH INTERNATIONAL 2014.(2014)