In this study, hydroxyethyl chitosan (HECTS), a water-soluble derivative of chitosan, was used to create a blend membrane and its function and application as a scaffold in repair of mechanically damaged corneal epethelia were examined. The results showed that HECTS significantly promoted growth of corneal epithelial cells (CEpCs) in vitro and that CEpCs grew well on the HECTS-based blend membrane. Fluorescent imaging showed that CEpCs were interconnected and formed layers on the membrane. After transplanting the CEpCs-seeded membranes onto the damaged corneal epithelium, we found that the epithelium was repaired faster compared with control. The repaired corneal epithelium with the membrane had a more compact structure and a smoother surface than control when observed by histology and scanning electron microscope. These results demonstrate an ability of the tissue-engineered scaffold to speed up the repair of mechanically damaged corneal epithelium.
基金：
National High Technology Research and Development Program of
China (863 Program, Grant Nos. 2006AA02A132, 2007AA091603).
The authors thank Professor Zhiqiang Qu (Central laboratory, The
Affiliated Hospital of Qingdao University) for critically reviewing
this manuscript.
第一作者机构：[1]Qingdao Univ, Affiliated Hosp, Cent Lab, Qingdao 266003, Peoples R China
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Liang Ye,Xu Wenhua,Han Baoqin,et al.Tissue-engineered membrane based on chitosan for repair of mechanically damaged corneal epithelium[J].JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE.2014,25(9):2163-2171.doi:10.1007/s10856-014-5248-7.
APA：
Liang, Ye,Xu, Wenhua,Han, Baoqin,Li, Na,Zhao, WeiWei&Liu, Wanshun.(2014).Tissue-engineered membrane based on chitosan for repair of mechanically damaged corneal epithelium.JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE,25,(9)
MLA：
Liang, Ye,et al."Tissue-engineered membrane based on chitosan for repair of mechanically damaged corneal epithelium".JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE 25..9(2014):2163-2171
