机构：[1]Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmology & Visual Sciences Key Laboratory, No 1# Dong Jiao Min Xiang Dongcheng District, Beijing 100730, China首都医科大学附属北京同仁医院首都医科大学附属同仁医院[2]State Key Laboratory of Brain and Cognitive Sciences, Institute of Biophysics, Chinese Academy of Sciences, 15 Da-tun Road, Beijing 100101, China[3]Department of Ophthalmology, University of California, San Francisco School of Medicine, San Francisco, CA 94143, USA[4]Department of Anatomy & Neurobiology, Dalhousie University, Halifax, Nova Scotia, Canada
A small percentage of retinal ganglion cells (RGCs) express melanopsin and are intrinsically photosensitive (ipRGCs). Whether light can affect the development of ipRGCs is not clear. In the rat retina, we found constant light exposure during the first postnatal week significantly increased the number of melanopsin immunopositive ipRGCs. This increase was durable and specific for melanopsin immunopositive ipRGCs. BrdU labeling showed no proliferation of the melanopsin immunopositive ipRGCs during constant light exposure. Retrograde labeling from the superior colliculus showed that no other types of RGCs were induced to express melanopsin. Light exposure was effective in increasing melanopsin immunopositive ipRGCs only when it coincided with the apoptotic phase of RGC development. However, daily intravitreous injection of tetrodotoxin, blocking action potentials, abolished the light induced increase of melanopsin immunopositive ipRGCs. These findings indicate that early light exposure can increase the number of melanopsin immunopositive ipRGCs through a process dependent on intrinsic photosensitive spiking activity. Furthermore, the increase of melanopsin immunopositive ipRGCs is potentially induced by apoptosis suppression in ipRGCs or enhanced expression of melanopsin. (C) 2013 Elsevier Ltd. All rights reserved.
基金：
NSFCNational Natural Science Foundation of China (NSFC) [30530280, 30672283, 30973261]; National Basic Research Program of ChinaNational Basic Research Program of China [2007CB512208, 2006CB911003]; NSFC-CIHR China/Canada Joint Health Research Initiative Program [30611120529]
第一作者机构：[1]Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmology & Visual Sciences Key Laboratory, No 1# Dong Jiao Min Xiang Dongcheng District, Beijing 100730, China
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Hong Jie,Zeng Qiang,Wang Huaizhou,et al.Controlling the number of melanopsin-containing retinal ganglion cells by early light exposure[J].EXPERIMENTAL EYE RESEARCH.2013,111:17-26.doi:10.1016/j.exer.2013.03.011.
APA：
Hong, Jie,Zeng, Qiang,Wang, Huaizhou,Kuo, Debbie S.,Baldridge, William H.&Wang, Ningli.(2013).Controlling the number of melanopsin-containing retinal ganglion cells by early light exposure.EXPERIMENTAL EYE RESEARCH,111,
MLA：
Hong, Jie,et al."Controlling the number of melanopsin-containing retinal ganglion cells by early light exposure".EXPERIMENTAL EYE RESEARCH 111.(2013):17-26
