An internally controlled real-time PCR assay based on SYBR Green was developed to screen Klebsiella pneumoniae carbapenemases (KPCs) gene containing bacteria and was validated for clinical strains or surveillance specimens. When 248 clinical samples were tested, the sensitivity and specificity of the assay were 100% and 99%, respectively. (c) 2012 Elsevier B.V. All rights reserved.
第一作者机构:[1]Capital Med Univ, Beijing Tongren Hosp, Dept Lab Med, Beijing 100730, Peoples R China
通讯作者:
通讯机构:[1]Capital Med Univ, Beijing Tongren Hosp, Dept Lab Med, Beijing 100730, Peoples R China[*1]Capital Med Univ, Beijing Tongren Hosp, Dept Lab Med, 1 Dongjiaominxiang Rd, Beijing 100730, Peoples R China
推荐引用方式(GB/T 7714):
Wang Lijun,Gu Haitong,Lu Xinxin.Rapid low-cost detection of Klebsiella pneumoniae carbapenemase genes by internally controlled real-time PCR[J].JOURNAL OF MICROBIOLOGICAL METHODS.2012,91(3):361-363.doi:10.1016/j.mimet.2012.09.009.
APA:
Wang, Lijun,Gu, Haitong&Lu, Xinxin.(2012).Rapid low-cost detection of Klebsiella pneumoniae carbapenemase genes by internally controlled real-time PCR.JOURNAL OF MICROBIOLOGICAL METHODS,91,(3)
MLA:
Wang, Lijun,et al."Rapid low-cost detection of Klebsiella pneumoniae carbapenemase genes by internally controlled real-time PCR".JOURNAL OF MICROBIOLOGICAL METHODS 91..3(2012):361-363