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Construction and delivery of gene therapy vector containing soluble TNF alpha receptor-IgGFc fusion gene for the treatment of allergic rhinitis

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机构: [1]China CDC, Inst Viral Dis Control & Prevent, Beijing 100052, Peoples R China [2]Beijing Tong Ren Hosp, Beijing, Peoples R China
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关键词: soluble TNF receptor allergic rhinitis EBV-based vector gene therapy

摘要:
Tumor necrosis factor a plays primary role in the pathogenesis of inflammatory diseases. TNFot is essential for antigen-specific IgE production and for the induction of Th2-type cytokines. The lack of TNF alpha inhibited the development of allergic rhinitis. In this study, the chimeric gene of soluble TNF receptor and IgGFc fragment (sTNFR-IgGFc) was cloned into the EBV-based plasmid pGEG. When the plasmid pGEG-sTNFR-IgGFc was transferred to endothelium cell, a considerable expression of the sTNFR-IgGFc fusion protein was detected. Moreover, the expression product in the supernatant, could antagonize the cytolytic activity of TNFot on L929 cells. Then the plasmid was delivered into nasal mucosa of allergic rhinitis mice to determine its effect on this animal model. Results showed that symptoms in treated group were improved. Pathological examination showed the numbers of eosinophil, mast cell and IL-5(+) cells in treated groups were reduced compared with placebo group. These data showed that pGEG-sTNFR-IgGFc expression plasmid is potential for the treatment of allergic rhinitis, and suggest that the antagonist of TNFa may provide a new approach for the treatment of allergic rhinitis. (c) 2007 Elsevier Ltd. All rights reserved.

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出版当年[2005]版:
大类 | 3 区 生物
最新[2023]版:
大类 | 3 区 医学
小类 | 3 区 生化与分子生物学 3 区 细胞生物学 3 区 免疫学
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出版当年[2004]版:
Q3 CELL BIOLOGY Q3 IMMUNOLOGY Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
最新[2023]版:
Q2 CELL BIOLOGY Q2 IMMUNOLOGY Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

影响因子: 最新[2023版] 最新五年平均 出版当年[2004版] 出版当年五年平均 出版前一年[2003版] 出版后一年[2005版]

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第一作者机构: [1]China CDC, Inst Viral Dis Control & Prevent, Beijing 100052, Peoples R China
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