机构：[1]Beijing Institute of Ophthalmology, TongRen Eye Center, TongRen Hospital, Capital University of Medical Sciences, China研究所眼科研究所首都医科大学附属北京同仁医院首都医科大学附属同仁医院[2]Beijing Institute of Ophthalmology and the Beijing TongRen Eye Bank, TongRen Eye Center, Beijing TongRen Hospital, Capital University of Medical Sciences, China研究所眼科研究所首都医科大学附属北京同仁医院首都医科大学附属同仁医院[3]Department of Immunology, General Hospital of PLA, China[4]Centre Laboratory, Beijing TongRen Hospital, Capital University of Medical Sciences, China首都医科大学附属北京同仁医院首都医科大学附属同仁医院[5]Academy of Military Medical Sciences, China
Aims: To explore the role of staphylococcal enterotoxin B (SEB) in treating high risk corneal keratoplasty in rats. Methods: Rat corneal high risk transplantation rejection models were set up using Fisher 344 and Lewis rats. The experimental rats were injected intraperitoneally with 0.2 ml SEB at different concentrations before keratoplasty. The rejection indexes of the allograft were recorded and the lymphocyte infiltration in the allograft and the percentage of the lymphocyte subpopulation in the lymphatic organs were also examined. Lymphocyte proliferation ability and the concentration of IL-2 and IL-10 in the serum were also evaluated. Results: Compared with the control group, SEB prolonged the survival time of the allograft significantly from 7 to 12 days. It could also reduce CD4(+) and CD8(+) lymphocyte infiltration in the allograft and minimise the percentage of CD4(+) and CD8(+) lymphocytes in the lymphatic organs. The lymphocyte proliferation ability was also weakened. However, the percentage of CD4(+) INK T lymphocytes in the lymphatic organs was raised. The serum concentration of IL-10 was higher but IL-2 was lower in the SEB treated groups. Conclusions: SEB prolonged the survival time of the allograft in high risk rat corneal allo-transplantation, which may be caused by T cell deletion and acquisition of non-specific tolerance.
基金：
The work was supported by the National Natural Science Foundation
of China under project no 3071385.
第一作者机构：[1]Beijing Institute of Ophthalmology, TongRen Eye Center, TongRen Hospital, Capital University of Medical Sciences, China
通讯作者：
通讯机构：[2]Beijing Institute of Ophthalmology and the Beijing TongRen Eye Bank, TongRen Eye Center, Beijing TongRen Hospital, Capital University of Medical Sciences, China[*1]Beijing Institute of Ophthalmology and the Beijing TongRen Eye Bank, Beijing TongRen Eye Centre, Beijing TongRen Hospital, Capital University of Medical Sciences, China
推荐引用方式(GB/T 7714)：
Jie Y,Pan Z,Chen Y,et al.Non-specific tolerance induced by staphylococcal enterotoxin B in treating high risk corneal transplantation in rats[J].BRITISH JOURNAL OF OPHTHALMOLOGY.2005,89(3):364-368.doi:10.1136/bjo.2004.048959.
APA：
Jie, Y,Pan, Z,Chen, Y,Wei, Y,Zhang, W...&Xu, L.(2005).Non-specific tolerance induced by staphylococcal enterotoxin B in treating high risk corneal transplantation in rats.BRITISH JOURNAL OF OPHTHALMOLOGY,89,(3)
MLA：
Jie, Y,et al."Non-specific tolerance induced by staphylococcal enterotoxin B in treating high risk corneal transplantation in rats".BRITISH JOURNAL OF OPHTHALMOLOGY 89..3(2005):364-368
