It is very important to determine if recombinant adenoviral vector (Ad) can damage the auditory hair cells in guinea pig cochlea after transgene expression. In this study, the scanning electron microscope was used to determine if there was loss of the auditory hair cells after Ad.LacZ (Ad5 containing Escherichia coli galactosidase) was inoculated into the cochlea through the round window membrane. Seven days later all inner and outer hair cells were found to express the LacZ gene. Except for the sparse loss of outer hair cells in the basal turn and the second turn, there was insignificant loss in the other turns. All inner hair cells were present. The damage to auditory hair cells resulting from intracochlear inoculation of Ad is limited, and this vector can be used as one of the ideal delivery tools in gene therapy of the cochlea. (C) 2004 Elsevier B.V. All rights reserved.
基金：
This work was supported by the Beijing Natural Science Fund (No. 7002010).
第一作者机构：[1]Capital Univ Med Sci, Tongren Hosp, Dept Otorhinolaryngol, Beijing 100730, Peoples R China[2]Beijing Inst Otorhinolaryngol, Beijing 100005, Peoples R China
通讯作者：
通讯机构：[1]Capital Univ Med Sci, Tongren Hosp, Dept Otorhinolaryngol, Beijing 100730, Peoples R China[2]Beijing Inst Otorhinolaryngol, Beijing 100005, Peoples R China[*1]Capital Univ Med Sci, Tongren Hosp, Dept Otorhinolaryngol, 2 Chongnei St, Beijing 100730, Peoples R China
推荐引用方式(GB/T 7714)：
Demin Han,Zilong Yu,Erzhong Fan,et al.Morphology of auditory hair cells in guinea pig cochlea after transgene expression[J].HEARING RESEARCH.2004,190(1-2):25-30.doi:10.1016/S0378-5955(04)00020-6.
APA：
Demin Han,Zilong Yu,Erzhong Fan,Chan Liu,Sha Liu...&Zhongyan Liu.(2004).Morphology of auditory hair cells in guinea pig cochlea after transgene expression.HEARING RESEARCH,190,(1-2)
MLA：
Demin Han,et al."Morphology of auditory hair cells in guinea pig cochlea after transgene expression".HEARING RESEARCH 190..1-2(2004):25-30
