机构：[1]Chinese PLA General Hospital, Beijing 100039, China[2]Department of Ophthalmology, the 306th Hospital of PLA, Beijing 100101, China[3]Institute of Microbiology, Chinese Academy of Science, Beijing 100101, China[4]Anhui Medical University, Hefei 230000, Anhui Province, China[5]ThirdMilitaryMedicalUniversity, Chongqing 400038, China[6]The First Hospital of Jilin University, Changchun 130021, Jilin Province, China
To evaluate the effect of long-term weightlessness on retina and optic nerve in tail-suspension (TS) rats.A stimulated weightlessness model was established by suspending rats' tail. After 12wk, the ultrastructure and the number of optic nerve axons were observed by transmission electron microscope. The number of survival retinal ganglion cells (RGCs) was calculated by fluorescent gold retrograde labeling. Retina cells apoptosis was detected by TUNEL staining. The function of optic nerve and retina was evaluated by the visual evoked potential (VEP) and oscillatory potentials (Ops).The optic nerve axons were swollen and sparsely aligned, and the lamellar separation and myelin disintegration occurred after 12wk in TS rats. The density of optic nerve axons was 32.23±3.92 (vs 37.43±4.13, P=0.0145), the RGCs density was 1645±46 cells/mm(2) (vs 1867±54 cells/mm(2) P=0.0000), the incidence rate of retinal cells apoptosis was 5.38%±0.53% (vs 4.75%±0.54%, P=0.0238), the amplitude of VEP-P100 was 15.43±2.14 µV (vs 17.67±2.17 µV, P=0.0424), the latency of VEP-P100 was 69.05±5.34ms (vs 62.43±4.87ms P=0.0143) and the sum amplitude of Ops was 81.05±8.34 µV (vs 91.67±10.21 µV, P=0.0280) in TS group and the control group, respectively.Long-term weightlessness can induce the ultrastructural changes and functional depress of the optic nerve, as well as retinal cell damages in TS rats.
基金：
Supported by the National Natural Scientific
Foundation of China (No. 81271016).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2015]版：
大类|4 区医学
小类|4 区眼科学
最新[2025]版：
大类|4 区医学
小类|3 区眼科学
第一作者：
第一作者机构：[1]Chinese PLA General Hospital, Beijing 100039, China[2]Department of Ophthalmology, the 306th Hospital of PLA, Beijing 100101, China
共同第一作者：
通讯作者：
通讯机构：[2]Department of Ophthalmology, the 306th Hospital of PLA, Beijing 100101, China[*1]Department of Ophthalmology, the 306th Hospital of PLA, Beijing 100101, China.
推荐引用方式(GB/T 7714)：
Zhao Hong-Wei,Zhao Jun,Hu Lian-Na,et al.Effect of long-term weightlessness on retina and optic nerve in tail-suspension rats.[J].International Journal of Ophthalmology.2016,9(6):825-30.doi:10.18240/ijo.2016.06.06.
APA：
Zhao Hong-Wei,Zhao Jun,Hu Lian-Na,Liang Jing-Nan,Shi Yuan-Yuan...&Luo Ling.(2016).Effect of long-term weightlessness on retina and optic nerve in tail-suspension rats..International Journal of Ophthalmology,9,(6)
MLA：
Zhao Hong-Wei,et al."Effect of long-term weightlessness on retina and optic nerve in tail-suspension rats.".International Journal of Ophthalmology 9..6(2016):825-30
