To probe the relationship between the density of L-type calcium channels on outer hair cells(OHCs) among various turns and the tonotopic map in the guinea pig cochlea.The L-type calcium channels in outer hair cells of guinea pigs were labeled with DM-BODIPY DHP and RH414 and monitored using a laser scanning confocal microscope. The fluorescence ratio of DM-BODIPY DHP/RH414 revealed the distribution of L-type calcium channels on the cellular membrane.The results showed that the cellular membrane had lesser DM-BODIPY DHP staining when pretreated with nifedipine, indicating that calcium channels on OHCs had the specificity of a L-type calcium channel. The density of L-type calcium channels had a tendency of increasing gradually from low frequency areas to high frequency areas. Variance analysis showed that the difference of the density of L-type calcium channels on OHCs between the fourth turn and the second turn was significant. There were no apparent differences in the distribution of L-type calcium channels among various regions on the cellular membrane of OHCs.The descripency of the distribution of L-type calcium channels on OHCs among various turns of the guinea pig cochlea may be one of the molecular mechanisms of the tonotopic map of the cochlea.
基金：
国家自然科学基金资助项目 ( 39970 787)；
语种：
中文
PubmedID：
第一作者：
第一作者机构：[1]解放军总医院耳鼻咽喉-头颈外科解放军耳鼻咽喉科研究所
通讯作者：
推荐引用方式(GB/T 7714)：
郭英,苏振伦,杨伟炎,等.豚鼠耳蜗各回外毛细胞L型钙通道的分布[J].Zhonghua Er Bi Yan Hou Ke Za Zhi.2002,37(5):321-3.
