机构：[1]Beijing Institute of Otolaryngology.研究所耳鼻咽喉科研究所首都医科大学附属北京同仁医院首都医科大学附属同仁医院
出处：
ISSN：
摘要：
To explore the potential use of p53 in gene therapy for laryngeal cancer.A human laryngeal cancer cell line Hep-2 was used. Recombinant cytomegalovirus-promoted adeno-viruses containing human wild-type p53 cDNA was transiently introduced into Hep-2 cells in vitro and injected into tumor nodules in vivo. The growth of Hep-2 cells in vitro and established s.c. squamous carcinoma nodules in nude mice was examined.The transduction efficiency of Hep-2 cell line was 100% at > or = 100 MOI. The p53 protein expression peaked on day 2 after infection and lasted far 5 days. Cell growth was greatly suppressed. In vivo studies, Ad5CMV-p53 transfestion in vitro inhibited tumorigenicity of Hep-2 cells in nude mice. Intra-tumoral injection of Ad5CMV-p53 significantly inhibited established s.c. implanted xenograft.Transfection of wild-type p53 gene via Ad5CMV-p53 is a potential approach to the therapy of laryngeal cancer.
语种：
中文
PubmedID：
第一作者：
第一作者机构：[1]Beijing Institute of Otolaryngology.
推荐引用方式(GB/T 7714)：
Wang Q,Han D,Wang W.Adenovirus-mediated p53 gene therapy of human laryngeal cancer[J].Chinese Journal of Oncology.1998,20(6):418-21.
