机构：[1]Department of Pathology, Xuzhou Medical University, Xuzhou 221000, China.[2]Discipline of Pathology, Bosch Institute and School of Medical Sciences, Charles Perkins Center D17, Sydney Medical School, The University of Sydney, Sydney, NSW 2006, Australia.[3]Department of Surgery, Songjiang Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 201600, China.[4]Tongren Hospital, Shanghai Jiaotong University, School of Medicine, Shanghai 200336, China.
Background Gastric cancer (GC) is a malignancy with high morbidity/mortality, partly due to a lack of reliable biomarkers for early diagnosis. It is important to develop reliable biomarker(s) with specificity, sensitivity and convenience for early diagnosis. The role of tumour-associated macrophages (TAMs) and survival of GC patients are controversial. Macrophage colony stimulating factor (MCSF) regulates monocytes/macrophages. Elevated MCSF is correlated with invasion, metastasis and poor survival of tumour patients. IL-34, a ligand of the M-CSF receptor, acts as a "twin" to M-CSF, demonstrating overlapping and complimentary actions. IL-34 involvement in tumours is controversial, possibly due to the levels of M-CSF receptors. While the IL-34/M-CSF/M-CSFR axis is very important for regulating macrophage differentiation, the specific interplay between these cytokines, macrophages and tumour development is unclear. Methods A multi-factorial evaluation could provide more objective utility, particularly for either prediction and/or prognosis of gastric cancer. Precision medicine requires molecular diagnosis to determine the specifically mutant function of tumours, and is becoming popular in the treatment of malignancy. Therefore, elucidating specific molecular signalling pathways in specific cancers facilitates the success of a precision medicine approach. Gastric cancer tissue arrays were generated from stomach samples with TNM stage, invasion depth and the demography of these patients (n = 185). Using immunohistochemistry/histopathology, M-CSF, IL-34 and macrophages were determined. Results We found that IL-34 may serve as a predictive biomarker, but not as an independent, prognostic factor in GC; M-CSF inversely correlated with survival of GC in TNM III-IV subtypes. Increased CD68(+)TAMs were a good prognostic factor in some cases and could be used as an independent prognostic factor in male T3 stage GC. Conclusion Our data support the potency of IL-34, M-CSF, TAMs and the combination of IL-34/TAMs as novel biological markers for GC, and may provide new insight for both diagnosis and cellular therapy of GC.
基金：
This study was supported by Grants from National Natural Science Foundation
of China (NO. 81502030) for the reagents and histopathology and immunohistochemistry,
and The Bosch small equipment grands SB), the University of
Sydney, Australia for image analysis. Liu was a recipient of a Travel fellowship,
Minster of Education, Jiangsu Province, China (QL) sponsored Dr. Liu’s stipend
during her stay in Australia.
第一作者机构：[1]Department of Pathology, Xuzhou Medical University, Xuzhou 221000, China.[2]Discipline of Pathology, Bosch Institute and School of Medical Sciences, Charles Perkins Center D17, Sydney Medical School, The University of Sydney, Sydney, NSW 2006, Australia.
通讯作者：
通讯机构：[2]Discipline of Pathology, Bosch Institute and School of Medical Sciences, Charles Perkins Center D17, Sydney Medical School, The University of Sydney, Sydney, NSW 2006, Australia.[4]Tongren Hospital, Shanghai Jiaotong University, School of Medicine, Shanghai 200336, China.
推荐引用方式(GB/T 7714)：
Liu Qinghua,Zhang Ying,Zhang Jiwei,et al.Inverse correlation between Interleukin-34 and gastric cancer, a potential biomarker for prognosis[J].CELL AND BIOSCIENCE.2020,10(1):doi:10.1186/s13578-020-00454-8.
APA：
Liu, Qinghua,Zhang, Ying,Zhang, Jiwei,Tao, Kun,Hambly, Brett D.&Bao, Shisan.(2020).Inverse correlation between Interleukin-34 and gastric cancer, a potential biomarker for prognosis.CELL AND BIOSCIENCE,10,(1)
MLA：
Liu, Qinghua,et al."Inverse correlation between Interleukin-34 and gastric cancer, a potential biomarker for prognosis".CELL AND BIOSCIENCE 10..1(2020)
生物学