机构：[1]Department of Neurosurgery, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200062[2]Department of Orthopedic Surgery, Tongren Hospital, School of Medicine, Shanghai JiaoTong University, Shanghai 200331, P.R. China
The hypoxic state of the brain tissue surrounding craniocerebral injury induces an increase in the secretion of HIF-1 alpha during the healing process. HIF-1 alpha can promote mesenchymal stem cell (MSC) migration to ischemic and hypoxic sites by regulating the expression levels of molecules such as stromal cell-derived factor-1 (SDF-1) in the microenvironment. Stem cells express the SDF-1 receptor C-X-C chemokine receptor type 4 (CXCR4) and serve a key role in tissue repair, as well as a number of physiological and pathological processes. The present study aimed to determine the role of HIF-1 alpha /SDF-1/CXCR4 signaling in the process of accelerated fracture healing during craniocerebral injury. Cultured MSCs underwent HIF-1 alpha knockdown to elucidate its effect on the proliferative ability of MSCs, and the effect of SDF-1 in MSCs was investigated. It was also determined whether HIF-1 alpha could promote osteogenesis via SDF-1/CXCR4 signaling and recruit MSCs. The results indicated that HIF-1 alpha knockdown suppressed MSC proliferation in vitro, and SDF-1 promoted cell migration via binding to CXCR4. Furthermore, HIF-1 alpha knockdown inhibited MSC migration via SDF-1/CXCR4 signaling. Considering the wide distribution and diversity of roles of SDF-1 and CXCR4, the present results may form a basis for the development of novel strategies for the treatment of craniocerebral injury.
基金：
Shanghai Excellent Young Medical Talents Training Program [2018YQ46]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81501897]; Shanghai Municipal Commission of Health and Family Planning of Science and Research Fund [20154Y0070]; K.C. Wong Education Foundation
第一作者机构：[1]Department of Neurosurgery, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200062
通讯作者：
通讯机构：[2]Department of Orthopedic Surgery, Tongren Hospital, School of Medicine, Shanghai JiaoTong University, Shanghai 200331, P.R. China[*1]Department of Orthopedic Surgery, Tongren Hospital, School of Medicine, Shanghai JiaoTong University, 1111 Xianxia Road, Shanghai 200331, P.R. China
推荐引用方式(GB/T 7714)：
Xue Yonghua,Li Zhikun,Wang Yi,et al.Role of the HIF-1 alpha/SDF-1/CXCR4 signaling axis in accelerated fracture healing after craniocerebral injury[J].MOLECULAR MEDICINE REPORTS.2020,22(4):2767-2774.doi:10.3892/mmr.2020.11361.
APA：
Xue, Yonghua,Li, Zhikun,Wang, Yi,Zhu, Xiaodong,Hu, Ruixi&Xu, Wei.(2020).Role of the HIF-1 alpha/SDF-1/CXCR4 signaling axis in accelerated fracture healing after craniocerebral injury.MOLECULAR MEDICINE REPORTS,22,(4)
MLA：
Xue, Yonghua,et al."Role of the HIF-1 alpha/SDF-1/CXCR4 signaling axis in accelerated fracture healing after craniocerebral injury".MOLECULAR MEDICINE REPORTS 22..4(2020):2767-2774
