机构：[1]Department of Biological Sciences, Purdue University, West Lafayette, Indiana, United States.[2]Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts, United States.[3]Department of Biochemistry, Purdue University, West Lafayette, Indiana, United States.[4]Purdue University Center for Cancer Research, Purdue University, West Lafayette, Indiana, United States.[5]Purdue University Libraries, Purdue University, West Lafayette, Indiana, United States.[6]Center for Statistical Science, Tsinghua University, Beijing, China.[7]Department of Statistics, University of Georgia, Athens, Georgia, United States.[8]Department of Ophthalmology and Visual Sciences, Chinese University of Hong Kong, Hong Kong.[9]Joint Shantou International Eye Center, Shantou University and the Chinese University of Hong Kong, Shantou, China.[10]Department of Biochemistry and Molecular Biology, Indiana University School of Medicine Lafayette, West Lafayette, Indiana, United States.[11]Purdue Institute for Integrative Neuroscience, Purdue University, West Lafayette, Indiana, United States.[12]Purdue Institute for Drug Discovery, Purdue University, West Lafayette, Indiana, United States.
Although zebrafish rods begin to develop as early as 2 days postfertilization (dpf), they are not deemed anatomically mature and functional until 15 to 21 dpf. A recent study detected a small electroretinogram (ERG) from rods in a cone mutant called no optokinetic response f (nof) at 5 dpf, suggesting that young rods are functional. Whether they can mediate behavioral responses in larvae is unknown.We first confirmed rod function by measuring nof ERGs under photopic and scotopic illumination at 6 dpf. We evaluated the role of rods in visual behaviors using two different assays: the visual-motor response (VMR) and optokinetic response (OKR). We measured responses from wild-type (WT) larvae and nof mutants under photopic and scotopic illuminations at 6 dpf.Nof mutants lacked a photopic ERG. However, after prolonged dark adaptation, they displayed scotopic ERGs. Compared with WT larvae, the nof mutants displayed reduced VMRs. The VMR difference during light onset gradually diminished with decreased illumination and became nearly identical at lower light intensities. Additionally, light-adapted nof mutants did not display an OKR, whereas dark-adapted nof mutants displayed scotopic OKRs.Because the nof mutants lacked a photopic ERG but displayed scotopic ERGs after dark adaptation, the mutants clearly had functional rods. WT larvae and the nof mutants displayed comparable scotopic light-On VMRs and scotopic OKRs after dark adaptation, suggesting that these responses were driven primarily by rods. Together, these observations indicate that rods contribute to zebrafish visual behaviors as early as 6 dpf.
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出版当年[2019]版：
大类|2 区医学
小类|2 区眼科学
最新[2023]版：
大类|2 区医学
小类|2 区眼科学
第一作者：
第一作者机构：[1]Department of Biological Sciences, Purdue University, West Lafayette, Indiana, United States.
共同第一作者：
通讯作者：
通讯机构：[1]Department of Biological Sciences, Purdue University, West Lafayette, Indiana, United States.[9]Joint Shantou International Eye Center, Shantou University and the Chinese University of Hong Kong, Shantou, China.[10]Department of Biochemistry and Molecular Biology, Indiana University School of Medicine Lafayette, West Lafayette, Indiana, United States.[11]Purdue Institute for Integrative Neuroscience, Purdue University, West Lafayette, Indiana, United States.[12]Purdue Institute for Drug Discovery, Purdue University, West Lafayette, Indiana, United States.[*1]Joint Shantou International Eye Center, Dong Xia North Road, Shantou, 515041, Guangdong Province, China[*2]Department of Biological Sciences, Purdue University, 915 W. State Street, West Lafayette, IN, 47907, USA
推荐引用方式(GB/T 7714)：
Venkatraman Prahatha,Mills-Henry Ishara,Padmanabhan Karthik Ramaswamy,et al.Rods Contribute to Visual Behavior in Larval Zebrafish.[J].Investigative ophthalmology & visual science.2020,61(12):11.doi:10.1167/iovs.61.12.11.
APA：
Venkatraman Prahatha,Mills-Henry Ishara,Padmanabhan Karthik Ramaswamy,Pascuzzi Pete,Hassan Menna...&Leung Yuk Fai.(2020).Rods Contribute to Visual Behavior in Larval Zebrafish..Investigative ophthalmology & visual science,61,(12)
MLA：
Venkatraman Prahatha,et al."Rods Contribute to Visual Behavior in Larval Zebrafish.".Investigative ophthalmology & visual science 61..12(2020):11
医学