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Linear epitope landscape of the SARS-CoV-2 Spike protein constructed from 1,051 COVID-19 patients.

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收录情况： ◇ SCIE

作者：
Li Yang[1,5] * ; Ma Ming-Liang[1] * ; Lei Qing[2] * ; Wang Feng[3] * ; Hong Wei[4,6] * ; Lai Dan-Yun[1] ; Hou Hongyan[3] ; Xu Zhao-Wei[1] ; Zhang Bo[3] ; Chen Hong[1] ; Yu Caizheng[7] ; Xue Jun-Biao[1] ; Zheng Yun-Xiao[1] ; Wang Xue-Ning[1] ; Jiang He-Wei[1] ; Zhang Hai-Nan[1] ; Qi Huan[1] ; Guo Shu-Juan[1] ; Zhang Yandi[2] ; Lin Xiaosong[2] ; Yao Zongjie[2] ; Wu Jiaoxiang[8] ; Sheng Huiming[8] ; Zhang Yanan[4,6] ; Wei Hongping[4,6] ; Sun Ziyong[3] ; Fan Xionglin[2] ; Tao Sheng-Ce[1] ;
机构： [1]Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University,Shanghai, China [2]Department of Pathogen Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology,Wuhan, China [3]Department of Clinical Laboratory, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China [4]CAS Key Laboratory of Special Pathogens and Biosafety, Centre for Biosafety Mega-Science, Wuhan Institute of Virology, Chinese Academyof Sciences, Wuhan, Hubei, China [5]College of Life Science, Nankai University, Tianjin 300071, China [6]University of Chinese Academy of Sciences, Beijing, China [7]Department of Public Health, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China [8]Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
出处：
DOI： 10.1016/j.celrep.2021.108915
ISSN：

摘要：
To fully decipher the immunogenicity of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Spike protein, it is essential to assess which part is highly immunogenic in a systematic way. We generate a linear epitope landscape of the Spike protein by analyzing the serum immunoglobulin G (IgG) response of 1,051 coronavirus disease 2019 (COVID-19) patients with a peptide microarray. We reveal two regions rich in linear epitopes, i.e., C-terminal domain (CTD) and a region close to the S2' cleavage site and fusion peptide. Unexpectedly, we find that the receptor binding domain (RBD) lacks linear epitope. We reveal that the number of responsive peptides is highly variable among patients and correlates with disease severity. Some peptides are moderately associated with severity and clinical outcome. By immunizing mice, we obtain linear-epitope-specific antibodies; however, no significant neutralizing activity against the authentic virus is observed for these antibodies. This landscape will facilitate our understanding of SARS-CoV-2-specific humoral responses and might be useful for vaccine refinement. Copyright © 2021 The Author(s). Published by Elsevier Inc. All rights reserved.

基金：
the National Key Research and Development Program of China grant (no. 2016YFA0500600), the Science and Technology Commission of Shanghai Municipality (no. 19441911900), the Interdisciplinary Program of Shanghai Jiao Tong University (no. YG2020YQ10), the National Natural Science Foundation of China (nos. 31970130, 31600672, 31670831, and 31370813), and China Postdoctoral Science Foundation (no. 2020M680857).

基金编号： 2016YFA0500600 19441911900 YG2020YQ10 31970130 31600672 31670831 31370813 2020M680857

语种：
外文
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中科院(CAS)分区：
出版当年[2020]版：
大类 | 1 区 生物
小类 | 2 区 细胞生物学
最新[2025]版：
大类 | 1 区 生物学
小类 | 2 区 细胞生物学
JCR分区：
出版当年[2019]版：
Q1 CELL BIOLOGY
最新[2023]版：
Q1 CELL BIOLOGY

影响因子： 最新[2023版] 最新五年平均 出版当年[2019版] 出版当年五年平均 出版前一年[2018版] 出版后一年[2020版]

第一作者：
第一作者机构： [1]Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University,Shanghai, China [5]College of Life Science, Nankai University, Tianjin 300071, China
共同第一作者：
通讯作者：
通讯机构： [4]CAS Key Laboratory of Special Pathogens and Biosafety, Centre for Biosafety Mega-Science, Wuhan Institute of Virology, Chinese Academyof Sciences, Wuhan, Hubei, China [6]University of Chinese Academy of Sciences, Beijing, China
推荐引用方式(GB/T 7714)：
Li Yang,Ma Ming-Liang,Lei Qing,et al.Linear epitope landscape of the SARS-CoV-2 Spike protein constructed from 1,051 COVID-19 patients.[J].CELL REPORTS.2021,34(13):doi:10.1016/j.celrep.2021.108915.
APA：
Li Yang,Ma Ming-Liang,Lei Qing,Wang Feng,Hong Wei...&Tao Sheng-Ce.(2021).Linear epitope landscape of the SARS-CoV-2 Spike protein constructed from 1,051 COVID-19 patients..CELL REPORTS,34,(13)
MLA：
Li Yang,et al."Linear epitope landscape of the SARS-CoV-2 Spike protein constructed from 1,051 COVID-19 patients.".CELL REPORTS 34..13(2021)

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