机构：[1]Beijing Key Laboratory of Nasal Diseases, Beijing Institute of Otolaryngology,Beijing, China研究所耳鼻咽喉科研究所首都医科大学附属北京同仁医院首都医科大学附属同仁医院[2]Department of Otolaryngology‑Head and Neck Surgeryand Department of Allergy, Beijing Tongren Hospital, Capital Medical University,Beijing, China临床科室耳鼻咽喉-头颈外科变态反应科首都医科大学附属北京同仁医院首都医科大学附属同仁医院[3]Upper Airways Research Laboratory, ENT Department,Ghent University, Ghent, Belgium[4]Department of Immunology, Schoolof Basic Medical Science, Capital medical University, Beijing, China
Mugwort and house dust mite (HDM) are two of the most common inhalant allergens in Asia, however, whether mugwort affects polysensitized HDM+ allergic rhinitis (AR) patients has not been elucidated.Overall, 15,884 AR outpatients were assessed for clinical status. Amino acid sequences of mugwort were determined by mass spectrometry. Afterward, cross-reactivity between mugwort tropomyosin and Dermatophagoides pteronyssinus 10 (Der p10) was analysed by ELISA inhibition and basophil activation experiments. To compare immunologic responses eliciting by two different tropomyosins, peripheral blood mononuclear cells (PBMCs) of HDM-monosensitized patients were stimulated by mugwort, HDM, Der p10 and synthetic peptides representing mugwort tropomyosin respectively.Polysensitized HDM+AR patients were mainly sensitized to cat and mugwort, and the positive rate of monosensitized HDM+AR out-clinic patients was increased during the mugwort pollen season. Tropomyosin protein was able to find in mugwort. Synthetic tropomyosin peptide of mugwort activated basophils which were primed by HDM-specific IgE; ELISA inhibition experiment showed synthetic tropomyosin peptide of mugwort inhibited IgE binding to HDM tropomyosin, Der p10. Unlike HDM and Derp 10, mugwort and mugwort tropomyosin mainly induced IFN-γ and IL-17 release in PBMCs of monosensitized HDM+AR patients, but not IL-5.Pan-allergen tropomyosin accounts for the cross-reactivity between mugwort and HDM, which reminds HDM+ patients to reduce mugwort exposure in mugwort pollen season in virtue of the tropomyosin induced mild inflammation.
基金：
Beijing Nova Program of
Science and Technology (Z191100001119117); Young top-notch talent
(2018000021223ZK12); the Beijing Hospitals Authority Youth Programme
(QML20180201); Beijing Scientific and Technological overall plan
(Z171100000117002 and Z181100001618002); the Beijing advanced innovation
center for food nutrition and human health (Beijing Technology and
Business University, 20181045); and the Program for the Changjiang Scholars
and Innovative Research Team (IRT13082).
语种：
外文
PubmedID：
第一作者：
第一作者机构：[1]Beijing Key Laboratory of Nasal Diseases, Beijing Institute of Otolaryngology,Beijing, China[2]Department of Otolaryngology‑Head and Neck Surgeryand Department of Allergy, Beijing Tongren Hospital, Capital Medical University,Beijing, China
共同第一作者：
通讯作者：
通讯机构：[1]Beijing Key Laboratory of Nasal Diseases, Beijing Institute of Otolaryngology,Beijing, China[2]Department of Otolaryngology‑Head and Neck Surgeryand Department of Allergy, Beijing Tongren Hospital, Capital Medical University,Beijing, China
推荐引用方式(GB/T 7714)：
Duan Su,Zhao Limin,Zhang Yuling,et al.Tropomyosin in mugwort cross-reacts to house dust mite, eliciting non-Th2 response in allergic rhinitis patients sensitized to house dust mite.[J].Clinical and molecular allergy : CMA.2021,19(1):2.doi:10.1186/s12948-021-00142-z.
APA：
Duan Su,Zhao Limin,Zhang Yuling,Zhang Nan,Zheng Ming...&Lan Feng.(2021).Tropomyosin in mugwort cross-reacts to house dust mite, eliciting non-Th2 response in allergic rhinitis patients sensitized to house dust mite..Clinical and molecular allergy : CMA,19,(1)
MLA：
Duan Su,et al."Tropomyosin in mugwort cross-reacts to house dust mite, eliciting non-Th2 response in allergic rhinitis patients sensitized to house dust mite.".Clinical and molecular allergy : CMA 19..1(2021):2
