机构：[1]Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University, Shanghai, China[2]Department of Pathogen Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China[3]Department of Clinical Laboratory, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China华中科技大学同济医学院附属同济医院[4]Key Laboratory of Gastrointestinal Cancer (Fujian Medical University), Ministry of Education, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, China[5]Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China[6]Department of Public Health, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China华中科技大学同济医学院附属同济医院
The immunogenicity of the SARS-CoV-2 proteome is largely unknown, especially for non-structural proteins and accessory proteins. In this study, we collect 2,360 COVID-19 sera and 601 control sera. We analyze these sera on a protein microarray with 20 proteins of SARS-CoV-2, building an antibody response landscape for immunoglobulin (Ig)G and IgM. Non-structural proteins and accessory proteins NSP1, NSP7, NSP8, RdRp, ORF3b, and ORF9b elicit prevalent IgG responses. The IgG patterns and dynamics of non-structural/accessory proteins are different from those of the S and N proteins. The IgG responses against these six proteins are associated with disease severity and clinical outcome, and they decline sharply about 20 days after symptomonset. In non-survivors, a sharp decrease of IgG antibodies against S1 and N proteins before death is observed. The global antibody responses to non-structural/accessory proteins revealed here may facilitate a deeper understanding of SARS-CoV-2 immunology.
基金：
This work was
partially supported by the National Key Research and Development Program
of China (grant no. 2016YFA0500600) and the National Natural Science Foundation of China (31970130, 31600672, 31670831, 31370813, 31900112,
and 21907065).
第一作者机构：[1]Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University, Shanghai, China
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Yang Li,Zhaowei Xu,Qing Lei,et al.Antibody landscape against SARS-CoV-2 reveals significant differences between non-structural/accessory and structural proteins[J].CELL REPORTS.2021,36(2):doi:10.1016/j.celrep.2021.109391.
APA：
Yang Li,Zhaowei Xu,Qing Lei,Dan-yun Lai,Hongyan Hou...&Sheng-ce Tao.(2021).Antibody landscape against SARS-CoV-2 reveals significant differences between non-structural/accessory and structural proteins.CELL REPORTS,36,(2)
MLA：
Yang Li,et al."Antibody landscape against SARS-CoV-2 reveals significant differences between non-structural/accessory and structural proteins".CELL REPORTS 36..2(2021)
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